利用小麦微卫星标记定位一个来自野生二粒小麦的抗白粉病基因  

Molecular Tagging of a Major Powdery Mildew Resistance Gene MlG in Wheat Derived from Wild Emmer by Using Microsatellite Marker

作　　者：解超杰[1] 倪中福[1] 孙其信[1] 杨作民[1] 刘保申[1] 魏艳玲[1] 

机构地区：[1]中国农业大学作物学院,北京100094

出　　处：《Acta Genetica Sinica》2001年第11期1034-1039,共6页

基　　金：中以农业研究基金 (SIARF)项目;北京市科委项目 (95 1 5 0 1 80 0 )

摘　　要：培育抗病品种是控制小麦白粉病危害最经济有效而又安全的手段。寻找和创造新抗源是抗病育种的基础工作 ,是解决抗源单一化问题的有效途径。来自以色列的野生二粒小麦G - 30 5 -M对北京地区小麦白粉菌流行小种 1 5号表现免疫 ,用G - 30 5 -M与小麦品种 781杂交并用京 41 1回交 (G - 30 5 -M 781 京 41 1 3) ,成功地将G - 30 5 -M的抗白粉病基因转入普通小麦中。遗传分析表明转入小麦中的抗病性苗期表达受一对显性基因控制 ,该基因暂定名为MlG。用 96对小麦微卫星引物对一个 1 67株的抗性分离家系进行了SSR分析 ,发现引物WMS5 70扩增产物在抗感个体间存在多态性。经分离群体验证 ,抗病基因MlG与小麦染色体 6AL上的微卫星位点Xgwm5 70连锁 ,遗传距离为 1 4.9± 3.0cM ,据此将MlG定位于 6AL。根据系谱和基因位点分析 ,推断MlG基因是不同于已知抗白粉病基因的一个新基因。Powdery mildew caused by Erysiphe graminis f. sp. tr itici is one of the most important wheat diseases in many regions of the world . Breeding for resistant cultivars has been proved to be an effective and enviro nmentally safe method to control diseases in wheat production. It is necessary t o search for more resistance genes for the diversification of resistance genes i n wheat breeding. An Isreali wild emmer wheat (Triticum dicoccoides) accessi on 'G-305-M' was found resistant to the prevailing E. graminis f. sp. tr itici isolate Race No. 15 in Beijing region. The powdery mildew resistance has been transferred from G-305-M into common wheat by crossing and backcrossing (G -305-M/781//Jing 411*3). Genetic analysis showed that the resistance was contr olled by a single dominant gene at the seedling stage. A segregating BC 2F 3 f amily of the cross 'G-305-M/781//Jing 411*3' with 167 plants was chosen for SSR analysis. Totally 96 wheat microsatellite primer pairs were screened, only o ne primer pair WMS570 could generate polymorphic DNA fragments between the resis tant and susceptible plants. After evaluating this polymorphic marker in the seg regating population, the microsatellite locus Xgwm570 mapped on chromosome 6 AL was found to be linked to the resistance gene, with the estimated genetic dis tance of 14.9±3.0 cM. Based on the origin and chromosomal location of the gene, it is suggested that the resistance gene derived from G-305-M should be a novel Pm gene and is temporarily designated MlG.

关 键 词：小麦 野生二粒小麦 抗白粉病基因 微卫星标记 基因定位 

分 类 号：S512.1[农业科学—作物学]