猪脾细胞干扰素与人白细胞干扰素之间的抗原性关系  

Antigenic Relationship Between Porcine Spleen Cell Interferon and Human Leucocyte Interferon

作　　者：黄文林[1] 杨学楼 刘虹[1] 罗经[1] 孙松柏[1] 王汉中[1] 吴晰莹 

机构地区：[1]中国科学院武汉病毒研究所

出　　处：《病毒学杂志》1989年第2期193-198,共6页

摘　　要：我们应用中和试验在不同细胞上对干扰素血清与猪脾细胞干扰素(PSIFN)及人干扰素(HuIFN-α)的中和能力进行了试验,结果证明HuIFN-α多克隆抗体具有明显的中和PSIFN的能力。而人免疫干扰素(Hu IFN-γ)抗体具有微弱的中和猪脾细胞干扰素能力。猪脾细胞干扰素不仅可在同源细胞表现出抗病毒活性,而且可在异源细胞表现出较高的抗病毒活性。应用人白细胞干扰素多克隆抗体亲和层析可有效地分离猪脾细胞干扰素,证实猪脾细胞干扰素与人白细胞干扰素之间具有高度的同源性关系。The antigenic relationship between interferon prepared in porcine spleen cells (PS) and human interferon was investigated. The result showed that the PsIFN can be neutralized not only by anti-HuIFN-α sera but also by anti-HuIFN-γ, sera, the ability of neutralization of anti-HuIFN-α was higher than that of anti-HuIFN-γ sera. The antiviral activities of the porcine spleen cell interferon can be expressed on both homologous species cells and heterologous species cells. The porcine spleen cell interferon was subjected to affinity chromatography on Sepharose-bound globulins from sheeps immunized with human leucocyte interferon. The porcine spleen cell interferon was efficiently absorbed and eluted from such affinity column. It was demonstrated that there is a high degree of antigenic cross reaction between porcine spleen cell interferon and human leucocyte interferon.

关 键 词：干扰素 猪脾细胞 血清学关系 

分 类 号：R392.11[医药卫生—免疫学]