盐胁迫下盐藻特异表达基因片段的克隆  

Cloning of Expressing cDNA Segments in Dunaliella Salina (Chlorophaceae) Under Salt Stress

作　　者：张晓宁[1] 万由衷[1] 林长发[1] 张亚兰[2] 李彦舫[2] 沈大棱[1] 

机构地区：[1]复旦大学生命科学学院遗传学研究所,上海200433 [2]中国人民解放军农牧大学,长春130062

出　　处：《复旦学报（自然科学版）》2001年第5期500-503,共4页Journal of Fudan University：Natural Science

基　　金：上海市科委基金项目;总后勤部军需部资助项目(960306)

摘　　要：盐碱、干旱、极端温度等逆境条件是影响植物生长的重要因素．以抑制消减杂交及差式库的筛选对盐藻（Ｄｕｎａｌｉｅｌｌａ　　ｓａｌｉｎａ）在高渗胁迫下特异表达基因片段进行了克隆．比较长期（１周，ＬＳ）及短期（１６　　ｈ，ＳＳ）ＮａＣＩ胁迫条件下基因表达情况，前者克隆到２个特异表达片段，后者也为２个．通过测序及ＧｅｎｅＢａｎｋ中进行同源搜索，均未有可靠的同源性结果．可以初步认为，以上得到的４个。ＤＮＡ可能是新基因或此片段不在保守区内．Adversity, such as salinity, aridity and extreme temperature, is an important factor to affect plant growth. In the present study, some cDNAs of the specially expressing genes of Dunaliella salina under hyperosmotic shock were cloned by suppression subtractive hybridization (SSH) and differentially screening of subtracted library. The differentially expression genes under salt stress for a longer phase (1 week, LS) were compared to those for a shorter phase( 16 hour, SS). Two cDNA clones were obtained from the former treatment and 3 clones from the latter treatment. After sequencing and homogeneity search in GeneBank, no reliable homogeneity results were found. It seems tentatively that they are probably novel genes or the fragments which are not located in the conservation domain.

关 键 词：盐藻 盐胁迫 抑制消减杂交 基因克隆 特异表达基因 真盐生物 基因表达 

分 类 号：Q949.2[生物学—植物学] Q785