用核酸序列测定1型糖尿病HLA-DPB1和DQB1第2外显子基因  

Sequencing-based typing of HLA-DPB1 and DQB1 exon 2 in patients with type 1 diabetes mellitus

作　　者：张胜兰[1] 邢万佳[1] 克丙申[1] 晏辉[1] 姜兆顺[1] 

机构地区：[1]济南军区总医院内分泌科,250031

出　　处：《中华医学遗传学杂志》2001年第5期362-365,共4页Chinese Journal of Medical Genetics

摘　　要：目的　研究山东省汉族 1型糖尿病与 HL A- DPB1和 HL A- DQB1等位基因的相关性。方法采用基于核酸序列测定的基因分型技术对 5 2例 1型糖尿病患者及 38例正常对照进行了 DPB1和 DQB1基因分析。结果　 DPB1* 2 2 0 1(P<0 .0 1)和 DQB1* 0 2 0 1(P<0 .0 1)、* 0 30 3(P<0 .0 5 )及 * 0 6 0 4(P<0 .0 5 )等位基因频率在糖尿病患者组显著高于对照组 ,而 DPB1* 0 40 2 (P<0 .0 1)和 DQB1* 0 30 1(P<0 .0 1)等位基因在糖尿病患者组显著低于对照组。　结论　DPB1* 2 2 0 1和 DQB1* 0 2 0 1、* 0 30 3及 * 0 6 0 4等位基因可能是山东省汉族 1型糖尿病的易感性等位基因 ,而 DPB1* 0 40 2和 DQB1* 0 30 1等位基因可能是山东省汉族Objective: To investigate the correlation between type 1 diabetes mellitus susceptibility and HLA-DPB1 and DQB1 genes in Shandong Han population. Methods: HLA-DPB1 and DQB1 genes analysis were performed by sequencing-based typing in 52 type 1 diabetes mellitus patients and 38 normal controls. Results: The frequency of DPB1 * 2201 in diabetics was significantly higher than that in controls (26.92% vs5.26%, P<0. 01), but the frequency of DPB1 * 0402 in diabetics was significantly lower than that in controls (11.54% vs 34.21%, P<0.01). The frequencies of DQB1 * 0201, * 0303 and * 0604 in diabetics were higher than those in controls (24.04% vs 10.53%, P<0.01; 22.12% vs6. 58%, P<0.05; 19.23% vs7.89%, P<0.05), while the frequency of DQB1 * 0301 in diabetics was significantly lower than that in controls (4.81% vs 25.00%, P<0.01). Conclusion: DPB1 * 2201, DQB1 * 0201, * 0303 and * 0604 may be susceptible alleles, and DPB1 * 0402 and DQB1 * 0301 may be protective alleles in patients with type 1 diabetes mellitus.

关 键 词：1型糖尿病 人类白细胞抗原DPB1基因 人类白细胞抗原DQB1基因 核酸序列 测定 

分 类 号：R587.1[医药卫生—内分泌]