腹主动脉瘤组织中基质金属蛋白酶的蛋白质水平研究  

THE CONTENTS AND DISTRIBUTION OF MATRIX METALLPROTEINASE IN RAT ABDOMINAL AORTIC ANEURYSMS

作　　者：冯翔[1] 景在平[1] 

机构地区：[1]第二军医大学长海医院,上海200433

出　　处：《解放军医学杂志》2001年第9期681-683,共3页Medical Journal of Chinese People's Liberation Army

基　　金：国家自然科学基金资助课题 (编号 3 9770 72 1)

摘　　要：为在蛋白质水平探讨基质金属蛋白酶 (MMP)在腹主动脉瘤组织中的含量及分布 ,将 40只健康雄性Wistar大鼠随机分为两组 ,实验组 2 0只大鼠以肾下腹主动脉加压灌注法建立腹主动脉瘤动物模型 ,于术后第 14天取肾下腹主动脉标本 ,对照组直接取肾下腹主动脉标本 ,中性甲醛固定 ,石蜡切片 ,分别做基质金属蛋白酶 2(MMP 2 )和基质金属蛋白酶 9(MMP 9)的免疫组化染色 ,染色后用计算机图像分析法进行基质金属蛋白酶的定位及相对定量研究。结果发现 ,实验组大鼠术后 14天腹主动脉扩张率大于 10 0 % ,MMP 2和MMP 9含量均明显高于正常腹主动脉 ,腹主动脉瘤瘤壁组织中中膜MMP 2含量明显高于MMP 9。提示MMP 2可能是腹主动脉瘤组织中细胞外基质 (ECM)To investigate the contents and distribution of matrix metallproteinases(MMP) in abdominal aortic aneurysm(AAA) on protein level, forty male Wistar rats were randomly divided into two groups, the rats of experimental group were perfused with pancreatic elastase in the isolated abdominal aorta to construct the model of AAA. Laparotomy was preformed on the 14th day after operation, the aortas were measured and harvested. The rats of control group were operated with the aortas harvested immediately. Immunohistochemical staining and computer image analysis were performed to analyze the contents and distribution of MMP 2 and MMP 9. The aortic diameter progressed to aneurysmal dimension in the experimental group. Immunohistochemistry study revealed that the MMP contents in AAA exceeded that in normal aorta obviously. The MMP 2 cotents in AAA tunica media were higher than MMP 9. It is suggested that MMP 2 plays the main role in extracellular matrix degradation of AAA tunica media.

关 键 词：腹主动脉瘤 基质金属蛋白酶 发病机制 MMP AAA 

分 类 号：R543.16[医药卫生—心血管疾病]