弹力蛋白在腹主动脉瘤发病中的作用  

THE ROLE OF ELASTIN IN EXPERIMENTAL ANIMAL MODEL OF ABDOMINAL AORTIC ANEURYSM

作　　者：冯翔[1] 景在平[1] 赵珺[1] 蔡国君[2] 

机构地区：[1]第二军医大学长海医院,上海200433 [2]第二军医大学解剖学教研室

出　　处：《解放军医学杂志》2001年第9期693-695,共3页Medical Journal of Chinese People's Liberation Army

基　　金：国家自然科学基金资助课题 (编号 3 9770 72 1)

摘　　要：为探讨腹主动脉中膜弹力蛋白结构和数量变化与腹主动脉瘤直径之间的关系 ,选取健康雄性Wis tar大鼠 45只 ,5只作为正常对照 ,其余随机分为 2组 ,实验组以肾下腹主动脉加压灌注法建立腹主动脉瘤动物模型 ,对照组灌注生理盐水。分别于术后即刻、2、7、14天取标本 ,测量腹主动脉直径 ,计算扩张率 ,组织固定特殊染色后做计算机图像分析 ,计算弹力蛋白的相对含量。结果发现 ,实验组大鼠腹主动脉在术后 2天开始明显扩张 ,至术后 14天腹主动脉扩张率与实验组差别有显著性意义 (P <0 0 5 ) ,腹主动脉中膜弹力蛋白含量与腹主动脉直径扩张率成直线负相关。提示 。To investigate the relationship between the diameter of abdominal aortic aneurysm(AAA) and the concentration of elastin, forty five healthy male Wistar rats were selected,5 rats of them were used as normal control, the other 40 rats were randomly divided into 2 groups. A 1 cm segment of the abdominal aorta of the rat was isolated and perfused with pancreatic elastase in the experimental group, and the control group was given normal saline solution. Laparotomy was performed immediately after operation and on the 2, 7, 14 day, the aortas were measured and harvested. Histological studies were carried out to analyze the changes in elastin. The aortic diameter progressed to aneurysmal dimension in the experimental group. Histological study revealed loss of elastin in tunica media of the aorta in the experimental group. Neither macroscopic nor microscopic lesions were found in control group. It is suggested that there is a significant correlation between the aortic diameter and the decreased elastin content.

关 键 词：腹主动脉瘤 弹力蛋白 发病机制 AAA 

分 类 号：R543.16[医药卫生—心血管疾病]