大孔树脂吸附法富集保和丸中橙皮苷的工艺研究  

Study on Enrichment Process of Hesperidin of Baohe Pills with Macroporous Resin

作　　者：刘中秋[1] 赖小平[1] 吴燕红[1] 蔡雄[1] 梁前[1] 

机构地区：[1]广州中医药大学,广东广州510405

出　　处：《中成药》2001年第8期550-552,共3页Chinese Traditional Patent Medicine

基　　金：广东省重点科技攻关项目 (中药有效成分提取分离技术研究 2 0 0 0 )

摘　　要：目的 :研究大孔树脂吸附法富集保和丸中有效成分的工艺条件及参数。方法 :以保和丸中主要药味陈皮中的主要成分橙皮苷富集程度为考察指标 ,考察大孔树脂富集保和丸有效成分的最佳工艺条件。结果 :保和丸提取液 (5 0 0mg/ml) 5mL上大孔树脂柱 (R15mm×H10 0mm) ,吸附 30min后 ,先用 10 0mL蒸馏水洗脱除去杂质 ,然后 ,采用 10 0mL 5 0 %乙醇洗脱橙皮苷为最佳工艺。结论 :通过大孔树脂富集橙皮苷洗脱率在 95 %以上 ,5 0 %乙醇洗脱液干燥后总固物约为处方量的 4%。Objective: To study the technological parameters of the enrichment purification process of Baohe Pills with macroporous resin. Methods: With the enriched degree of hesperidin of Pericarpium Citri Reticulatae of Baohe Pills as a marker, the optimum technological parameters of the enrichment process were studied. Results: The optimum process is that 5mL of the extract of BaoHe Pills (500mg/mL) was adsorbed for 30min with a column of macroporous resin (R15mm×H100mm) and the resin was washed with 100mL of distilled water, then the hesperidin was eluted from the macroporous resin with 100mL of 50% ethanol. Conclusion: The elutive rate of hesperidin was above 95% by means of the macroporous resin. So this process enriching the active components of Baohe Pills is feasible.

关 键 词：保和丸 橙皮苷 大孔树吸附法 富集 中成药 

分 类 号：R284[医药卫生—中药学]