高效液相色谱法测定血浆中平痛新的浓度  

Determination of the Concentration of Nefopam in Plasma by HPLC

作　　者：赵晓萌[1] 董淳[1] 徐群英[1] 常翠[1] 毕殿洲[2] 

机构地区：[1]深圳市人民医院,暨南大学附属第二医院药学部,深圳市518020 [2]沈阳药科大学药剂教研室,沈阳市110015

出　　处：《中国药房》2001年第6期350-357,共8页China Pharmacy

摘　　要：目的 :建立高效液相色谱法测定血浆中微量平痛新浓度的分析方法。方法 :采用HPLC法 ,色谱柱 :KromasilC18 柱 (Φ4 6mm×200mm ,5μm) ;流动相 :甲醇 -水 -三乙胺 (50∶50∶0 5) ;流速 :0 7ml/min ;检测波长 :215nm ;灵敏度 :0 04AUFS ;内标物 :奥美拉唑。结果 :血浆中平痛新浓度在10～600ng/ml范围内线性关系良好 ,相关系数r=0 9993 ;平均回收率为 (100 36±1 1) % ;平均日内和日间差异分别为4 7 %和3 8 % ;最低检测限可至1ng/ml。结论 :该法简便、稳定 ,可用于盐酸平痛新体内药代动力学与生物利用度研究。OBJECTIVE:To develop a method to determine the contents of nefopam in plasma by HPLC METHODS:RP-HPLC method,Kromasil C18 column(Φ4 6×200mm,5μm);mobile phase was methenol-water-triethylamine(50∶50∶0 5);Detecting wavelength was 215nm;The flow rate was 0 7ml/min;sensitivity was 0 04AUFS;HPLC internal standard was Omeprazole RESULTS:The calibration cure was linear in the concentrations ranged from 10ng/ml to 600ng/ml(r=0 9 993) The average recovery was (100 36±1 1)% The RSD within day was less than 4 7% and the RSD between days was less than 3 8% CONCLUSION:This method is simple and reliable,which also can be applied to the study of pharmacokinetics and bioavailability of nefopam in vivo

关 键 词：平痛新 高效液相色谱法 血浆药物浓度 

分 类 号：R97[医药卫生—药品]