测定人血清白蛋白的荧光光纤流动免疫分析系统  

Determination of Human Serum Albumin by Novel Flow- injection Fluorescence Immunoassay System with Fiber-optic Sensor

作　　者：何俊[1] 李勇[1] 但德忠[2] 李毓琦[1] 乔小蓉[1] 王方强[2] 

机构地区：[1]四川大学华西医学中心药学院,四川成都610041 [2]四川大学环境科学与工程学院,四川成都610056

出　　处：《分析测试学报》2001年第5期31-33,共3页Journal of Instrumental Analysis

基　　金：国家自然科学基金资助项目 (39570218)

摘　　要：用新型荧光光纤免疫传感流动分析测试系统 ,对人血清白蛋白 (HSA)进行测试 ;将偶联于微晶纤维素表面形成的HSA固相抗体与待测HSA(抗原 )、荧光标记抗原 (FITC_HSA)竞争性结合 ,经稀碱液处理 ,固相载体与抗原抗体复合物分离 ,用新型荧光光纤免疫传感流动分析系统测定解析液的荧光值 ,以求得待测HSA的含量 ;该法检出限为0.1g/L ,日内RSD0.91 %～6.4 % ,日间RSD1.8 %～8.0 %,加标回收率91 %～120 % ;用该法测定注射用HSA,与临床检验常用的溴甲酚绿法对照,具有良好的相关性 (r=0.9808)。A novel flow _ injection fluorescence immunoassay system with fiber _ optic sensor has been developed for the determination of human serum albumin(HSA). A solid phase antibody of HSA was prepared by linking HSA antibody to microcrystalline cellulose, then to it HSA and labeling HSA with a fluorescent tag were competitively bonded. The formed antigen-antibody complex was dealt with a dilute alkaline solution and separated from the solid support, the fluorescence yield was determined by the sys_tem. The experimental conditions were optimized. The detection limit of 0.1 g/L for HSA, intra _ day determinarion RSD of 0.91%～6.4%, inter _ day determination RSD of 1.8%～8.0% and recovery rate of 91%～120% were found. The analytical results of HSA obtained by proposed method were well related to those of clinical bromcresol green(BCG) method(r=0.980 8).em.Theexperimentalconditionswereoptimized.Thedetectionlimitof0.1g/LforHSA,intra_daydeterminarionRSDof0.91 %～6.4%,inter_daydeterminationRSDof1.8 %～8.0%andrecoveryrateof91 %～120 %werefound.TheanalyticalresultsofHSAobtainedbyproposedmethodwerewellrelatedtothoseofclinicalbromcresolgreen(BCG)met

关 键 词：荧光免疫分析 光纤传感器 流动注射分析 人血清白蛋白 临床检验 

分 类 号：R446.11[医药卫生—诊断学] R446.61[医药卫生—临床医学]