稀土离子和钙调蛋白对乳酸脱氢酶活性的影响  

Effects of Rare Earth Ions and Calmod ulin on the Activity of Lactate Dehydrogenase

作　　者：李伟国[1] 卢振伟[1] 赵大庆[1] 倪嘉缵[1] 

机构地区：[1]中国科学院长春应用化学研究所稀土化学与物理开放实验室,长春130022

出　　处：《无机化学学报》2001年第6期843-848,共6页Chinese Journal of Inorganic Chemistry

基　　金：国家自然科学基金资助项目(No.29890280)

摘　　要：分别研究了三价稀土离子、稀土离子和钙调蛋白CaM的复合物以及作为稀土离子配体的二乙三胺五乙酸DTPA及其衍生物二乙三胺五乙酸双二甲酰胺DTPABDMA和二乙三胺五乙酸双异烟肼DTPABIN对乳酸脱氢酶LDH活性的影响。结果表明稀土离子浓度小于3μmol·L-1时对酶活性具有轻微的激活作用离子浓度增大到6μmol·L-1后则开始表现出抑制作用钙调蛋白可以缓解这种抑制作用二乙三胺五乙酸及其衍生物在浓度超过5μmol·L-1时对酶活性均有抑制这种抑制作用在加入外源的钙调蛋白后也得到缓解。In this paper, the effects of rare earth ions (La3+, Eu3+, Dy3+, Yb3+) and their complexes with calmodulin on the activity of lactate dehydrogenase (LDH) were investigated. The results reveal that whether binding with calmodulin or not, rare earth ions show a minor activation effects on LDH when their concentrations are less than 3 mu mol (.) L-1, but indicate some strong inhibitory effects on LDH activity when the concentrations are above 5 mu mol (.) L-1. Calmodulin, which is a calcium-dependent regulator, can stimulate LDH activity and release the inhibitory effects of rare earth ion. Diethylenetriamine pentaacetic acid(DTPA) and its derivatives bisdimethylamide-diethylenetriamine pentaacetic acid (DTPA-BDMA), bisisonicotinyl-diethylenetriamine pentaacetic acid (DTPA-BIN), which are often used as ligands to metal ions, inhibit LDH activity when their concentrations are above 5 mu mol (.) L-1. Calmodulin can also release their inhibitory effects at the same time.

关 键 词：乳酸脱氢酶 钙调蛋白 稀土离子 二乙三胺五乙酸 酶活性 作用机理 抑制作用 

分 类 号：Q554[生物学—生物化学] O614.33[理学—无机化学]