采用2-氯腺苷改善MTT法检测淋巴细胞激活和杀伤功能  

Improvement of MTT assay by 2-chloroadenosine in activation test and cytotoxicity test of lymphocyte

作　　者：黄波[1] 冯作化[1] 张桂梅[1] 

机构地区：[1]华中科技大学同济医学院医学分子生物学研究室,武汉430030

出　　处：《中国免疫学杂志》2001年第11期579-581,共3页Chinese Journal of Immunology

基　　金：国家自然科学基金 (No .39970 32 2 )资助项目

摘　　要：目的 :研究 2 氯腺苷 ( 2 ClA)特异性杀伤巨噬细胞对MTT法检测淋巴细胞激活试验和细胞毒试验的影响。方法 :运用细胞培养技术 ,培养小鼠脾淋巴细胞和腹腔巨噬细胞。通过MTT法检测 2 ClA处理和未处理的淋巴细胞的激活以及特异性活化后对肿瘤细胞的杀伤 ,同时还检测 2 ClA对巨噬细胞的毒性作用。结果 :2 ClA对巨噬细胞有很强的杀伤作用 ,采用MTT法检测淋巴细胞的活化和杀伤活性时 ,2 ClA处理组的OD值要明显低于未经 2 ClA处理的对照组 (P <0 0 5 )。结论 :2 ClA通过特异性杀伤巨噬细胞可以消除巨噬细胞在MTT法检测淋巴细胞激活和杀伤试验中所产生的影响 ,使检测更为准确。Objective:To sutdy the effect of 2 chloroadenosine(2 ClA),which is specifically cytotoxic to macrophages,on MTT assay in activation test and cytotoxicity test of lymphocyte.Methods:Using cell culture technique,mouse splenic lymphocytes and peritoneal macrophages were cultured.Lymphocyte activation and specific cytotoxicity to tumor cells and toxicity of 2 ClA to macrophages were measured by MTT assay in the presence or absence of 2 ClA.Results:2 ClA had a strong cytotoxic effect on macrophages.When the activation test and cytotoxicity test of lymphocyte were measured by MTT assay,the optical density values of 2 ClA group was lower than that of control group,and statistic analysis showed P<0 05.Conclusion:With the specific cytotoxicity to macrophages,2 ClA can exclude the influence of macrophages to MTT assay in activation test and cytotoxicity test of lymphocyte,and more reliable results will be obtained.

关 键 词：2-氯腺苷 巨噬细胞 淋巴细胞 MTT法 细胞激活 细胞毒 杀伤试验 

分 类 号：R329.24[医药卫生—人体解剖和组织胚胎学]