慢性乙型肝炎PBMC凋亡及淋巴细胞亚群的检测  

The assay of apoptosis of PBMC and lymphocyte subsets in chronic hepatitis B

作　　者：李玲[1] 顾长海[1] 李欣[2] 颜杰[3] 

机构地区：[1]第三军医大学西南医院全军感染病研究所,重庆400038 [2]重庆医科大学儿童医院中心试验室,重庆400014 [3]第三军医大学卫生统计学教研室,重庆400038

出　　处：《中国免疫学杂志》2001年第11期618-621,共4页Chinese Journal of Immunology

摘　　要：目的 :了解慢性 /慢性重型乙型肝炎外周血淋巴细胞激活诱导细胞死亡 (AICD)现象的存在情况、各免疫细胞的状况、AICD与淋巴细胞状况的关系 ,以探讨乙型肝炎慢性化和重型化的机制。方法 :利用慢性、慢性重型乙型肝炎病人和健康献血员外周血单个核细胞 (PBMC)在PHA P刺激下培养 72h ,通过流式细胞仪检测PBMC的凋亡情况 ;采用流式细胞仪结合全自动血液分析仪对慢性、慢性重型乙型肝炎病人及正常对照组外周血各淋巴细胞亚群进行检测。结果 :慢性乙型肝炎组PBMC凋亡率高于慢性重型乙型肝炎组 ( P<0 0 1) ,高于正常对照组 ( P <0 0 1)。慢性乙型肝炎组总淋巴细胞百分率高于慢性重型乙型肝炎组 (P <0 0 1) ,慢性重型乙型肝炎组淋巴细胞数低于正常对照组 (P <0 0 1) ;CD3 +、CD3 +CD4 +细胞数低于正常对照组 (P <0 0 1) ,低于慢性乙型肝炎组 (P <0 0 5 ) ;CD3 +CD8+细胞数低于正常对照组 (P <0 0 5 ) ,低于慢性乙型肝炎组 (P <0 0 5 )。单核细胞百分率高于正常对照组 (P <0 0 1) ,高于慢性乙型肝炎组 (P <0 0 5 )。结论 :慢性乙型肝炎患者外周血淋巴细胞活化与凋亡共存 ,慢性重型乙型肝炎患者外周血淋巴细胞消耗严重 ,AICD参与乙型肝炎慢性化、重型化的发生机理。Objective:In order to explore the AICD happening in PBMC and the situation of peripheral blood lymphocyte subsets in chronic/ chronic severe hepatitis B Methods:The peripheral blood mononuclear cells of patients with chronic /chronic severe hepatitis B were cultured with PHA P for 72 h Then the apoptosis of PBMC was assayed by flow cytometry The peripheral blood lymphocyte subsets of patients with chronic /chronic severe hepatitis B were assayed by flow cytometry and automatic blood analyzer Results:The percentage of apoptotic PBMC in chronic hepatitis B group was higher than that in chronic severe hepatitis B group(P<0 01) and higher than that in normal control group(P<0 01) The percentage of lymphocyte in chronic hepatitis B group was higher than that in chronic severe hepatitis B group(P<0 01) The number of lymphocyte in chronic severe hepatitis B group was lower than that in normal control group(P<0 01) The numbers of CD3+?CD3+CD4+ lymphocyte in chronic severe hepatitis B group were lower than that in normal control group(P<0 01) and lower than that in chronic hepatitis B group(P<0 05) The number of CD3+CD8+ lymphocyte in chronic severe hepatitis B group was lower than that in chronic hepatitis B group (P<0 05) and lower than that in normal control group(P<0 05) The percentage of monocyte in chronic severe hepatitis B group was higher than that in chronic hepatitis B group(P<0 01) and higer than that in normal control group(P<0 05) Conclusion:Activation and apoptosis co existed in the peripheral blood lymphocyte of chronic hepatitis B The lymphocytes in chronic severe hepatitis B were exausted greatly AICD may involve in the pathogenesis of chronic and chronic severe hepatitis B

关 键 词：慢性乙型肝炎 激活 诱导 细胞凋亡 淋巴细胞亚群 PBMC 

分 类 号：R512.62[医药卫生—内科学]