丙型肝炎病毒NS5b区酶切基因分型及其在干扰素治疗中的应用  

Typing of hepatitis C virus(HCV)genome with restriction endonuclease and its predictive value on the response to interferon therapy.

作　　者：许青田[1] 杜绍财[2] 季颖[2] 王会民[1] 李新月[1] 陶其敏[2] 

机构地区：[1]郑州市第六人民医院,河南郑州450005 [2]北京医科大学人民医院肝病研究所,北京100044

出　　处：《临床肝胆病杂志》2001年第4期231-232,共2页Journal of Clinical Hepatology

摘　　要：探讨丙型肝炎病毒 (HCV)感染的基因型及其与干扰素应答效果的关系。应用逆转录 -聚合酶链反应(RT -PCR)技术对 2 6 9例肝炎患者血清进行HCVNS5b区基因片段扩增 ,并对 5 6例阳性PCR产物进行酶切分型 ,同时观察干扰素α - 2b对其中 48例慢性丙型肝炎的疗效。HCV单纯 1b型感染、1b基因变异及 2a型感染对干扰素的应答率分别为 70 % (14/ 2 0 )、9 5 % (2 / 2 1)、71 4% (5 / 7) ;其中完全应答分别为 45 % (9/ 2 0 )、4 8% (1/ 2 1)、5 7 1% (4/ 7) ;部分应答分别为 2 5 % (5 / 2 0 )、4 8% (1/ 2 1)、14 3 % (1/ 7)。 1b基因变异组同单纯 1b型及 2a型感染组比较有显著性差异 (P <0 0 1) ,而单纯 1b型感染组与 2a型感染组比较无明显差异 (P >0 0 5 )。HCV单纯 1b型感染或 2a型感染者对干扰素敏感 ,而 1b基因变异者干扰素治疗效果极差 ,可作为预测干扰素疗效的一项新的重要指标。To investigate the genotypes of HCV infection in Beijing areas and the relationship between HCV genotypes and the effectiveness of interferon(IFN)therapy,reverse transcription polymerase chain reaction(RT PCR)for NS5b region was used to detect HCV RNA in 269 cases.56 positive PCR products were typed by restriction endonuclease,and 48 chronic hepatitis C patients with different genotypes(20 with type 1b,21 with type 1b genic variation,7 with type 2a)were treated with recombinant interferon α 2b(IFN α 2b)for 24weeks.The response rate of HCV genotype 1b infection was 70%(14/20),1b genic variation co infection was 9.5%(2/21),2a infection was 71.4%(5/7).The response rate of HCV 1b or 2a alone was significantly higher than that of HCV 1b genic variation ( P <0.01).

关 键 词：丙型肝炎病毒 基因分型 聚合酶链反应 干扰素 NS56区酶切分型 

分 类 号：R512.63[医药卫生—内科学]