无血清培养基与完全培养基对体外诱导免疫细胞支持作用的比较  

Comparison of Serum-Free Medium AIMV with Standard Serum-Containing Medium in Culturing Cells Activated with IFN-γ,IL-2 and Anti-CD3

作　　者：孙婧[1] 陈红松[1] 高燕[1] 王松霞[1] 张毅[1] 王宇[1] 

机构地区：[1]北京大学人民医院肝病研究所,北京100044

出　　处：《中国肿瘤生物治疗杂志》2001年第3期208-212,共5页Chinese Journal of Cancer Biotherapy

基　　金：国家"973"项目资助 (编号G19990 5 410 6 ) ;国家"863"项目资助 (编号 10 2 12 0 2 0 7) ;国家自然科学基金重点项目资助 (编号 39830 42 0 )

摘　　要：目的 :多方面比较无血清培养基AIMV与完全培养基对体外诱导免疫细胞支持作用。方法 :分别用AIMV及完全培养基在IFN γ ,IL 2 ,及抗 CD3单抗存在的条件下培养人外周血单个核细胞 ,比较细胞的增殖能力、细胞表型、及细胞因子分泌能力 ,并比较不同培养基培养细胞回输体内后的抑制病毒效果。结果 :与完全培养基培养细胞相比 ,无血清培养基AIMV培养细胞增殖能力与之相当 ;CD2 5的表达率增高 ,表达持续时间延长 ;细胞因子IFN γ的分泌时间延长 ;回输体内后的抑制病毒作用更明显。结论 :无血清培养基AIMV用于培养临床治疗用的免疫细胞 ,综合效果优于完全培养基。Objective: To compare serum-free medium AIMV with standard serum-containing medium in culturing cells activated with IFN-γ,IL-2 and anti-CD3. Methods: Peripheral blood mononuclear cells (PBMC) separated from donors were cultured with IFN-γ,IL-2 and anti-CD3 in serum-free medium AIMV or in standard serum-containing medium separately. Proliferation, phenotypes and cytokine secretion of cells cultured in the two different media were compared. The inhibitory effects on hepatitis B virus replication in vivo were observed after transfusion of cells cultured in different media. Results: Compared with cells cultured in standard serum-containing medium, cells cultured in serum-free medium AIMV could proliferate well enough to be applied to immunotherapy; More percentage of cells cultured in AIMV expressed CD25; Cells cultured in serum-free medium secreted more IFN-γ; After transfusion of cells cultured in AIMV, hepatitis B replication could be inhibited more evidently. Conclusion: Serum-free medium AIMV was better than serum-containing medium in culturing cells for immunotherapy.

关 键 词：无血清培养基 免疫治疗 细胞表型 细胞因子 细胞培养 

分 类 号：R457.2[医药卫生—治疗学]