IL-12对慢性乙型肝炎患者T_(H1)/T_(H2)分化的影响  

Effect of IL-12 on T_(H1) /T_(H2)-type cytokines production in PBMC of chronic hepatitis B virus infection patients

作　　者：王少扬[1] 林玉梅[1] 马卫闽[1] 戚少然[2] 兰凤华[2] 

机构地区：[1]南京军区福州总医院传染科,福建福州350003 [2]南京军区福州总医院实验科,福建福州350025

出　　处：《免疫学杂志》2001年第6期466-468,共3页Immunological Journal

摘　　要：目的观察 IL- 12对慢性乙肝患者 TH1 / TH2 类细胞分化的影响。方法分离 5 0例慢性乙型肝炎患者 PBMC,分别与植物血凝素 (PHA ,10 0μg/ m L )、HBc Ag(1μg/ m L )、HBe Ag(1μg/ m L )单独或联合 IL - 12 (10 ng/ m L )体外培养 48h,EL ISA法检测培养上清液中 IL - 2、IFN -γ、IL - 4、IL - 10水平。 2 0例健康人群做对照。结果 IL - 12对健康人群 PBMC产生 TH1 /TH2 类细胞因子无显著影响 ,但对慢性乙型肝炎患者则显著增强 PBMC产生 IFN-γ,且慢性中度患者最为突出。 IL - 12与HBe Ag联合诱导 ,不但显著增强慢性乙型肝炎患者 PBMC产生 IL- 2和 IFN- γ,还抑制 IL- 4和 IL- 10的产生。结论 IL- 12可增强慢性乙型肝炎患者 IFN-γ优势表达 ,可促进 HBe Ag诱导的 TH2 型优势表达向 TH1ObjectiveTo investigate the effect of IL 12 on T H1 /T H2 type proliferation of chronic hepatitis B virus infection patients Methods PBMC of 50 individuals of chronic HBV infection were co cultured with PHA(100 μg/mL), HBcAg(1 μg/mL) ,HBeAg(1 μg/mL) in the absence or presence of rhIL 12(10 ng/mL) IL 2,IFN γ,IL 4,IL 10 production of PBMC were determined by ELISA ResultsIFN γ production was significantly increased in chronic hepatitis B virus infection patients in the presence of rhIL 12 On the other hand,IL 2 and IFN γ production were increased and IL 4 and IL 10 production decreased when PBMC of chronic hepatitis B virus infection patients were stimulated by HBeAg in presence of IL 12.Conclusion The data suggest that IL 12 may enhance IFN γ production in chronic HBV infection patients IL 12 can also change T H2 type proliferation by HBeAg induction into T H1 type proliferation

关 键 词：白细胞介素-12 乙型肝炎 外周血单个核细胞 TH1/TH2 

分 类 号：R512.62[医药卫生—内科学]