新生Balb/c鼠脑神经元和星形胶质细胞纯培养的实验研究  

Studies on neurons and astrocytes of newborn Balb/c mouse brain pure culture

作　　者：赵兰娟[1] 龚蕴贞[1] 林维芬[1] 胡涛[1] 张咏南[1] 

机构地区：[1]安徽医科大学微生物学教研室,合肥230032

出　　处：《安徽医科大学学报》2001年第4期251-254,共4页Acta Universitatis Medicinalis Anhui

摘　　要：目的　探讨纯培养神经元和星形胶质细胞的合适方法。方法　采用Balb/c鼠尾胶原、大鼠尾胶原、阿糖胞苷、贴壁处理及传代培养等方法纯培养Balb/c鼠神经元和星形胶质细胞。结果　纯培养Balb/c鼠神经元的纯度为 90 % ,纯培养星形胶质细胞的纯度为 97%。结论　纯培养神经元宜选择Balb/c鼠尾胶原为生长基质并经 3mg·L-1阿糖胞苷处理。纯培养星形胶质细胞宜用大鼠尾胶原为生长基质和培养Objective To seek suitable methods of neuron culture and a strocytes culture from newborn Balb/cmouse brain cortex. Methods Balb/c mouse tail collagen, rat tail collagen, cytosine arabinoside; adhesion treatment and subculture were adopted to culture neurons and astrocytes. Results The purity of neuron cultures was 90 percent, whereas the purity of astrocyte cultures was 97 percent. Conclusion Balb/c mouse tail collagen and cytosine arabinoside may be applied to culture neurons, whereas rat tail collagen, adhesion treatment and subculture may be applied to culture astrocytes.

关 键 词：神经元 星形胶质细胞 细胞培养 新生Balb/c鼠 

分 类 号：R329－33[医药卫生—人体解剖和组织胚胎学] R338[医药卫生—基础医学]