HCMV AD169株感染复数(MOI)的确定  

Determination of human cytomegalovirus AD169 strain multiplicity of infection

作　　者：韩俊[1] 王明丽[1] 姜永忠[1] 詹以森[1] 李艳秋[1] 余莉[1] 

机构地区：[1]安徽医科大学微生物学教研室,合肥230032

出　　处：《安徽医科大学学报》2001年第4期257-259,共3页Acta Universitatis Medicinalis Anhui

摘　　要：目的　通过蚀斑形成试验测定人巨细胞病毒(HCMV)AD16 9株的蚀斑 (PFU)确定其感染复数 (MOI)并纯化病毒。方法　HCMVAD16 9株接种 7～ 8代HF细胞单层 ,37℃吸附 ,0 0 0 7琼脂糖覆盖层 ,1周后加第 2层覆盖 ,2周后用 0 0 0 0 5结晶紫染色 5min ,记数 ,计算PFU及MOI;染色前 ,镜下挑取蚀斑保藏并鉴定。结果　蚀斑经 0 0 0 0 5结晶紫染色在HF细胞单层上呈紫色 ,很易识别 ,散在均匀分布 ,着色深 ,与周围区域界限明显 ,MOI =3 41× 10 4 。结论　探索出HCMVAD16 9株感染复数 (MOI)Objective To assay human cytomegalovirus multiplicity of infection (MOI). Methods HCMV AD169 strain was inocclated into 7～8 passages HF cells monolayer, adsorped in 37℃, added two agarose overlays within two weeks and stained with 0 000 5 crystal violet. Plaques were counted accurately. For reisolation of virus, plaques were removed with capillary pipettes before staining with 0 000 5 crystal violet. Results Plaques were stained darkly and distinguished easily from the normal HF cell layer; MOI=3 41×10 4. Conclusion Assay method of human cytomegalovirus MOI was found. MOI is a practical and accurate method for quantitation of HCMV AD169 strain.

关 键 词：巨细胞病毒属 分离 提纯 蚀斑 感染复数 HCMV MOI 

分 类 号：R512.99[医药卫生—内科学] R373.9[医药卫生—临床医学]