丙型肝炎患者外周血单个核细胞HCV免疫组化和电镜研究  

Immunohistochemical and electron microscopic study on the peripheral blood mononuclear cells in patients with chronic hepatitis C

作　　者：陈佩兰[1] 范公忍[1] 李琳[1] 田惠英[1] 陈良标[1] 

机构地区：[1]北京军区总医院全军肝病研究所,北京100700

出　　处：《第二军医大学学报》2001年第9期855-857,F003,共4页Academic Journal of Second Military Medical University

基　　金：军队医药卫生科研基金资助项目 ( 95 B0 13)

摘　　要：目的 :探讨丙型肝炎患者外周血单个核细胞 (PBMCs)内丙型肝炎病毒抗原 (HCVAg)表达和 HCV颗粒感染状况及意义。方法 :采用抗 HCV核心区及 NS3区单克隆抗体 ,对 35例慢性丙肝患者 PBMCs进行免疫组化检测和电镜观察。结果 :慢性丙肝患者 PBMCs的 HCVAg阳性率为 82 .9% (2 9/ 35 ) ,其中 82 .8% (2 4/ 2 9)的病例电镜下发现了病毒颗粒 ,占全组病例的 6 8.6 % (2 4/ 35 )。同时发现部分细胞超微结构呈现一系列凋亡性改变。结论 :HCV可感染 PBMCs并在其中复制 ,这可能是导致患者 HCV持续感染的原因之一。Objective: To study hepatitis C virus antigen (HCVAg) expression and hepatitis C virus (HCV) infection in peripheral blood mononuclear cells(PBMCs) in patients with chronic hepatitis C. Methods: The anti-HCV core and NS3 monoclonal antibodies with immunohistochemical staining and electron microscopy (EM) were applied to detect HCVAg and HCV in PBMCs of 35 patients. Results: Positive expression of HCVAg was 82.9% (29/35). It showed in EM that the positive rate of HCV particles was 82.8% (24/29) among 29 patients with HCVAg. It was 68.6% (24/35) of all specimens. The ultrastructural morphology with HCV infection in part of PBMCs had a series of apoptosis changes. Conclusion: HCV may infect the PBMCs and produce infectious HCV particles in PBMCs,as a result,it may be one of the reasons that HCV persistently infects patients.

关 键 词：丙型肝炎 单核白细胞 丙型肝炎病毒组 HCV 免疫组织化学 电镜 HCVAg 

分 类 号：R512.63[医药卫生—内科学]