病理性瘢痕中原癌基因c-myc和c-fos的表达  

Experimental study of the expression of c-myc and c-fos proto-oncogenes in hypertrophic and keloid scars

作　　者：胡振富[1] 罗力生[1] 

机构地区：[1]第一军医大学南方医院,广东广州510515

出　　处：《实用美容整形外科杂志》2001年第4期219-221,共3页Journal of Practical Aesthetic and Plastic Surgery

摘　　要：目的　探讨原癌基因的表达与病理性瘢痕形成的相关性。方法　应用免疫组化SP法 ,检测c -myc和c -fos蛋白在增生性瘢痕、瘢痕疙瘩和正常皮肤组织中的表达和分布 ,并用图像定量分析比较其差异。结果　在增生性瘢痕和瘢痕疙瘩的成纤维细胞中c-myc、c -fos呈强阳性表达 ,两组间无明显差异 ,而与正常皮肤对照组均有显著性差异。结论　增生性瘢痕与瘢痕疙瘩中c -myc、c -fos蛋白表达升高 ,存在c -myc和c -fos原癌基因的激活 ,可能参与了成纤维细胞的分化增殖或表型转化、胶原合成与降解以及对细胞因子的调控 ,并导致瘢痕增生。Background Abnormal scars, including hypertrophic and keloid scars, are the result of wound-over healing characterized by aberrant proliferation of fibroblasts and the accumulation of excessive collagen and extracellular matrix. The pathogenesis underlying the two lesions is unclear. Objective To investigate the correlation between the expression of key proto-oncogenes playing major roles in tumorogenic process and abnormal scarring. Methods Immunohistochemical technique was performed to detect the expression and distribution of c-myc and c-fos proteins in 100 samples biopsied from 10 cases of hypertrophic scars, 10 cases of keloids and 5 cases of normal skin with antibodies against c-myc and c-fos. Image analysis was applied to compare their quantitative difference of expression. Results c-myc and c-fos expression on hypertrophic and keloid scars was significantly higher than normal skin controls, and there was no difference between the two lesions. Positive expression distributed mainly in the nucleus of fibroblasts of the two lesions. Conclusion c-myc and c-fos oncogenes are activated in hypertrophic and keloid scars. They may contribute to proliferation and differentiation of fibroblasts, to synthesis and degradation of collagen and to regulation of cytokines or phenotype transformation of fibroblasts and may induce abnormal scarring. The mechanisms of their effects remain a mystery.

关 键 词：原癌基因蛋白质 基因表达 增生性瘢痕 瘢痕疙瘩 

分 类 号：R622[医药卫生—整形外科]