低强度He-Ne激光照射对K562细胞的抑制作用  

The Inhibitory Effects of Low Power He Ne Laser Irradiation on K562 Cells

作　　者：姚骏[1] 夏天骊[1] 吴旭峰[2] 高静[1] 赵晓宁[1] 胡正荣[2] 张祖暄[1] 

机构地区：[1]南京大学医学院,210093 [2]东南大学电子工程系

出　　处：《中国激光医学杂志》2001年第2期108-110,共3页Chinese Journal of Laser Medicine & Surgery

基　　金：国家自然科学基金资助项目! (6 95 780 0 4)

摘　　要：目的　观察不同能量密度的低强度He Ne激光对K5 6 2细胞的生物学作用。方法　以能量密度为 1 89、4 74和 9 47J/cm2 的He Ne激光照射K5 6 2细胞 ,检测细胞活性 ;以能量密度为 0 41、1 0 2和 2 0 3J/cm2 的He Ne激光照射K5 6 2细胞 ,测定超氧化物歧化酶 (SOD)活性。结果　中、高能量密度He Ne激光照射能显著抑制K5 6 2细胞生长 ,升高细胞内SOD活性 ,而低能量密度激光照射对细胞无明显影响。结论　较高能量密度的He Ne激光对K5 6 2细胞的生长有抑制作用 。Objective The biological effects of He Ne laser irradiation of different energy density on K562 cells were investigated to reveal the inhibitory effect of low intensity laser on K562 cells. Methods The inhibitory effects of 1 89, 4 74 and 9 47 J/cm 2 He Ne laser on K562 cell growth were studied by cell viability analysis. And the effects of 0 41, 1 02 and 2 03 J/cm 2 He Ne laser on superoxide dismutase(SOD) activity of K562 cells were also detected. Results K562 cell viability could be inhabited by middle and high energy density He Ne laser irradiations, and intracellular SOD activity could be increased at the same time. While no change occurred after low energy density laser exposure. Conclusions He Ne laser of higher energy density could inhibit the proliferation of K562 cells and promote intracellular SOD activity.

关 键 词：激光 K562医院 超氧化物歧化酶 肿瘤细胞 

分 类 号：R318.51[医药卫生—生物医学工程]