DNA疫苗诱导小鼠特异性细胞免疫及抗乙型肝炎病毒皮下移植瘤的研究  

Specific cellular immune responses and effect against subcutaneously transplanting tumor induced by DNA vaccine of HBV surface gene in mice

作　　者：杜德伟[1] 周永兴[1] 冯志华 李光玉[1] 姚志强[1] 

机构地区：[1]第四军医大学唐都医院全军感染病诊疗中心,西安710038

出　　处：《中华传染病杂志》2001年第4期216-218,共3页Chinese Journal of Infectious Diseases

基　　金：国家自然科学基金资助项目 ( 39770 665 )

摘　　要：目的　观察乙型肝炎病毒 (HBV)DNA疫苗 ( pCR3 .1 S)诱导Balb/c小鼠 (H 2 d)的特异性细胞免疫应答及其对稳定表达HBsAg的小鼠肥大细胞瘤P815细胞 (P815 HBV S) (H 2 d)成瘤性的影响。方法　肌肉注射DNA疫苗 ,背部皮下接种P815 HBV S细胞 ,观察成瘤情况 ,4h51Cr释放法检测小鼠脾细胞细胞毒T细胞 (CTL)杀伤活性。结果　DNA疫苗可以降低成瘤率 ,抑制肿瘤生长 ,延长小鼠平均存活期 ,提高小鼠存活率。CTL细胞杀伤活性明显增加 (P <0 .0 0 1)。结论　DNA疫苗可以诱导细胞免疫应答 ,对体内HBV感染可能具有预防及治疗作用。Objective To observe the specific immune responses and the protection against P815 mastocytoma cells stably expressing HBV surface antigen in H-2 d mice after DNA immunization of HBV surface antigen gene (pCR3.1-S). Methods The immunization was performed by intramuscular injection of DNA vaccine (pCR3.1-S). P815-HBV-S was inoculated subcutaneously into mice three weeks after DNA immunization. The tumor growth was measured every five days. HBsAg specific cytotoxic T lymphocyte (CTL) activity was measured by 51 Chromiunm release assay. Results HBV DNA vaccine can evidently inhibit the tumor growth, prolong the survival period and improve the survival rate in mice. Meanwhile, HBsAg specific CTL activity was obviously increased after DNA immunization. Conclusions The results show that the DNA vaccine, pCR3.1-S, has strong antigenecity in cellular immunity and has marked killing effect on HBV infected cells in vivo. DNA vaccine against HBV may be useful for both prophylactic and therapeutic purposes.

关 键 词：基因疫苗 乙型肝炎表面抗原 沣鼠 基因治疗 HBV DNA疫苗 

分 类 号：R512.62[医药卫生—内科学]