放射配基结合抑制分析监测抗β受体抗体应答  

A Radioligand Binding Inhibition Assay Monitoring Antibody Response Against β-Adrenergic Receptors

作　　者：李运有[1] 张寄南[1] 徐红[1] 马文珠[1] 王敬良[1] 李锦山[2] 

机构地区：[1]南京医学院第一附属医院心血管病研究室 [2]南京医学院第一附属医院中心实验室

出　　处：《南京医学院学报》1991年第2期83-85,共3页

摘　　要：通过正交试验改良放射配基(^(125)I-心得静)结合抑制分析(RBIA),所得的最佳实验条件为孵育温度30℃,缓冲液pH7.4,加血清预孵育40min,加^(125)I-PIN孵育20min;用于监测猪心肌细胞膜免疫BALB/c小鼠的抗β受体抗体应答,其血清中抗体对^(125)I-PIN结合β受体的抑制呈浓度依赖性,且为非竞争性抑制。同时还讨论了RBIA对于测定病人体内循环抗β受体自身抗体的临床应用价值。A radioligand binding inhibition assay (RBIA) was selected with widemodification to detect antibodies against adrenergic β-receptors in BALB/c mice immunizedwith purified swine cardiac membrane. In our laboratory ^(125)I-PIN labeled was used as theradioligand. The optimal experimental conditions obtained through the orthogonal analysisare as follows: preincubating cardiac membrane with diluted serum for 40 minutes, furtherincubating for 20 minutes after adding ^(125)I-PIN at 30℃ with pH 7.4 buffer. The resultsshowed that the immune sera inbibited the binding of ^(125)I-PIN to cardiac membrane in aconcentration-dependent and non-competitive manner. The clinical significance of thismethod in detecting circulating autoantibodies against β-adrenergic receptors in patients withheart diseases are discussed.

关 键 词：放射配基 抗β受体 自身抗体 

分 类 号：R446.62[医药卫生—诊断学]