应用PCR等核酸技术检测动物饲料中牛羊组织成分  

Species Specific PCR Detection of Bovine, Sheep, and Goat DNA in Animal Feedstuff

作　　者：陈茹[1] 林志雄[1] 刘琳琳[1] 

机构地区：[1]广州出入境检验检疫局,广东广州510623

出　　处：《中国兽医科技》2001年第9期3-8,共6页Chinese Journal of Veterinary Science and Technology

摘　　要：为准确鉴别不同品种动物源性饲料 ,防范疯牛病及羊痒病的传播 ,建立了检测牛、绵羊和山羊组织种间特异的聚合酶链反应 (PCR) ;用MboI、VspI、RsaI分别对从动物饲料中检测得到的牛、绵羊和山羊组织的PCR产物进行酶切分析 ,酶切图谱与序列结构相符 ;核酸测序结果表明 ,3种动物组织的PCR产物序列与基因库中检索到的相应序列相吻合。用 3种检测方法检测牛、绵羊和山羊组织无交叉反应 ,检测猪、鸡、兔和鱼等动物组织均呈阴性。PCR检测的敏感性可达0 .2 5 % (质量分数 ) ,PCR检测全过程包括样品处理 ,可在In order to accurate differentiation variety of animal feedstuff , control the spread of bovine spongiform encephalopathy and scrapie, a method has established to test for the presence of ruminant or mammalian materials in animal feedstuff.Three PCR assays were developed for respective detection and identification of bovine, sheep and goat specific mitochondrial DNA sequence from animal feeds. The specification of the testing assays were demonstrated by both restrition enzyme analysis & direct sequencing of amplified products from animal feedstuff. There was no cross-reaction among these three PCR assays. No PCR product was observed when testing DNA samples from fish and other vertebrates whose materials were commonly included in animal feedstuffs. The sensitivity of PCR detection was 0.25%. The whole examining procedure could completed within 3h, including 30min for DNA purificationand and 30min for gel electrophoresis.

关 键 词：核酸技术检测 动物源性饲料 PCR检测 动物组织 

分 类 号：S816.48[农业科学—饲料科学] S816.17[农业科学—畜牧学]