^(13)N-NH_3·H_2O的制备及生物分布研究  

Preparation and biodistribution of ^(13)N-ammonia

作　　者：王明芳[1] 唐刚华[1] 高晓[1] 李志[1] 陈育尧[2] 吴湖炳[1] 黄祖汉[1] 

机构地区：[1]广州第一军医大学南方医院,广州510515 [2]广州第一军医大学中医研究所,广州510515

出　　处：《核技术》2001年第11期920-924,共5页Nuclear Techniques

摘　　要：利用16O(p ,α) 13 N核反应 ,经戴氏合金 (Devarda’salloy)还原靶水制备出13 N -NH3 ·H2 O的生理盐水注射液。标记产物利用TLC、HPLC进行放射化学分析 ,放化纯度 >95 % ,放射性浓度为(1.11— 2 .2 2 )GBq/mL。小鼠分布实验显示心肌摄取率最高 ,平均 %ID/g为 19.5 4 % ;首次通过时 ,心肌和肺的摄取率最高 ,其 %ID/g各为 2 4 .90± 1.82和 2 2 .79± 1.94 ,约有 92 %的放射性从血液中清除 ;注射后 1min ,各器官中的放射性急剧下降 ,5min时下降到最低值 ,10min时各器官中的放射性又明显升高 ,在 1— 30min内 ,心脏摄取稳定在 11.34%— 2 9.2 4 %。13 N -NH3 ·H2 O有较高的心 /肝和心 /肺比值和较快的血液清除率。结果表明 ,Devarda’salloy还原法是一种简单快速、可获得较高核纯度和放化纯度的13 N -NH3 ·H2 O的制备方法。小鼠分布实验提示在静脉注射13 N-NH3 ·H2 O后 2— 5min内进行PET显像 ,可获得满意的图象。To study the synthetic method, quality control and biodistribution of 13 N-ammonia, 13 N-NO - 3 and 13 N-NO - 2 were produced from water via the 16 O(p,α) 13 N nuclear reaction with 16.5 MeV proton at 25-30 μA for 10-20 min and then converted to 13 N-ammonia by the Devarda's alloy. Radiochemical purity was better than 95% as determined by HPLC and TLC. The biodistribution study of 13 N-ammonia in mice indicated that the mean uptake of 13 N-ammonia in myocardium was as high as 19.54%. At the first-pass time, the uptakes of myocardial and lung were (24.90±1.82)% and (22.79±1.94)%, respectiviely, and 92% radioactivity was cleared from blood. After injection, the radioactivity in each organ was significantly readuced at 1 min and was minimum at 5min, but significantly increased at 10min. The uptake of myocardium varied within the range of 11.34-29.24 during 1-30min. High heart-to-liver and heart-to-lung ratios and rapid blood clearance were found. The results suggust that the Devarda's alloy method is a simple and rapid reducing means for the preparation of 13 N-ammonia. 13 N-ammonia was suitable for clinical regional blood perfusion imaging. PET imaging is suitable at 2-5min after injection.

关 键 词：^13N-NH3·H2O 制备 正电子药物 质量控制 生物分布 氨 心肌血流灌注显像 冠心病 诊断 氮13 放射性药物 标记物 

分 类 号：R817.42[医药卫生—影像医学与核医学]