互补DNA杂交分析和血清学方法对香石竹环斑病毒分离物的研究  

Studies on the Isolation of Carnation Ringspot Virus by cDNA Hybridization Analysis and Serology

作　　者：安德荣[1] 魏宁生[1] R.Koeni 

机构地区：[1]西北农业大学植保系 [2]联帮德国生物研究中心病毒所

出　　处：《病毒学杂志》1989年第4期408-414,共7页

摘　　要：在联帮德国奥卡河中分离到一种病毒分离物(W),通过鉴别寄主反应的测定,病毒粒子的电子显微镜观察,cDNA斑点杂交和血清学的研究,鉴定出这一病毒分离物为香石竹环斑病毒。其外壳蛋白亚基的分子量为3.8×10~4。cDNA吸印转移杂交分析表明,香石竹环斑病毒的两个RNA组份(RNA^1、RNA_2)之间没有核苷酸序列同源性。RNA_1和RNA_2分别由3.7和1.5仟碱基组成。A virus isolate (w) has been obtained from the Oker river in W.Germany. The isolate was identified as Carnation ringspot virus (CarRSV) by means of inoculation on the test plant, electron microscopy, dot blot hybridization and serology.The molecular weight of the coat protein is 3.8×10~4. Northern blot analysis revealed that the two RNA species of the virus which consisted of c. 3.7 and 1.5kb, respectively, but without base sequence homology.

关 键 词：香石竹 环斑病毒 互补DNA 血清学 

分 类 号：S432.41[农业科学—植物病理学]