高效液相色谱法测定格拉司琼的血药浓度  

Analysis of granisetron in human plasma by HPLC

作　　者：焦建杰[1] 邓岩[1] 张京玲[1] 娄建石[1] 张才丽[1] 董伟林[2] 

机构地区：[1]天津医科大学基础医学院药理学教研室,天津300070 [2]天津医科大学实验中心,天津300070

出　　处：《中国临床药学杂志》2001年第5期307-309,共3页Chinese Journal of Clinical Pharmacy

摘　　要：目的：建立人血浆中格拉司琼浓度的测定方法。方法：采用ＨＰＬＣ法，荧光检测器，色谱柱为Ｃ８柱（４．６ｍｍ×１５０ｍｍ，５μｍ）；流动相为乙腈－０．１ｍｏｌ／Ｌ醋酸钠ｐＨ ４．７（２５∶７５， ｖ／ｖ），内含１０ｍｍｏｌ／Ｌ己烷磺酸钠；荧光检测波长：Ｅｘ＝３０５ｎｍ、Ｅｍ＝３６０ｎｍ。血液样品用甲苯一次提取，吹干后残渣用流动相复溶，２０μｌ进样。结果：格拉司琼血药浓度标准曲线在０．１５６～４０ｎｇ／ｍｌ间呈线性相关，Ｙ＝５．６０７２ Ｘ—１４３７ ２（ｒ＝０．９９３ ３．ｒＳＮ＝３）。呈低检测限为０．１５６ ｎｇ／ｍｌ。方法的平均回收率为１０４．２％；日内及日间精密度均小于１０％。结论：本方法具有简便、灵敏、准确等优点，为该药物的监测与临床药理研究提供了分析方法。AIM: To establish HPLC (high performance liquid chromatography) method for deter- mining granisetron in human plasma. METHODS: Granisetron was extracted by toluene, then separated and determined by HPLC/fluorescence detection Ex= 305 nm, Em= 360 nm. RESULTS: The calibration curve was linear between 0. 156-40. 0 ng/mL with r=0. 993 3, and the limit detection was 0. 156 ng/mL. The average recovery was 104. 2%. RSD values of within-day and between day was not over 10%. CON- CLUSION: The present study provides a simple, accurate, and reliable method for pharmacokinetic studies as well as for determining concentration of granisetron in human plasma.

关 键 词：格拉司琼 高效液相色谱法 荧光检测 血药浓度 止吐药 

分 类 号：R969[医药卫生—药理学]