RP-HPLC法测定感冒清热颗粒中葛根素的含量  

RP-HPLC Determination of Puerarin in Ganmao Qingre Granules

作　　者：杨小平 余炳生[2] 饶燕平 郑冠彬 

机构地区：[1]湛江市药品检验所,524037 [2]湛江师范学院化学系,524048

出　　处：《药物分析杂志》2001年第5期331-333,共3页Chinese Journal of Pharmaceutical Analysis

摘　　要：目的 :建立高效液相色谱法测定感冒清热颗粒中葛根素含量。方法 :采用SuplcosilC16色谱柱 (15 0mm× 4 6mm ,5μm) ,甲醇 -水 (2 7∶73)为流动相 ,流速 1 0mL·min-1,检测波长为 2 48nm。结果 :葛根素在 0 2～ 2 0 0 μg·mL-1范围内 ,峰面积与其浓度线性关系良好 (r =0 9999) ,平均回收率为 10 1 7% ,RSD为 1 3 % (n =3)。结论 :本法简便、准确、重复性好 。Objective:To establish a HPLC method for determination of the content of puerarin in Ganmao Qing re granules.Methods:The condition of RP-HPLC was Suplcosil C 16 column(150 mm×4\^6 mm,5 μm),mobile phase of methanol-water (27∶73)with a flow rate of 1 0 mL·min -1 and UV detector at 248 nm.Results:The calibration curve was linear ( r =0 999 9)within the range of 0 2～200 μg·mL -1 for puerarin,the average recovery was 101 7%( n =3).Conclusion:This method is simple,rapid and reliable,suitable for the quality control of the drug.

关 键 词：葛根素 感冒清热颗粒 反相高效液相色谱法 含量测定 

分 类 号：R286.0[医药卫生—中药学] R284.1[医药卫生—中医学]