地塞米松抑制脊髓继发性损伤的实验研究  

THE INHIBITION OF THE SECONDARY SPINAL CORD INJURIES WITH DEXAMETHASONE: An experimental study

作　　者：杜良杰[1] 殷晓雪[2] 杨毓华 陈长安[1] 梅伟[1] 

机构地区：[1]河南省郑州市骨科医院,450052 [2]北京大学医学部临床第三附属医院 [3]陕西省中医学院附属医院

出　　处：《中医正骨》2001年第9期3-4,10,共3页The Journal of Traditional Chinese Orthopedics and Traumatology

摘　　要：为了研究地塞米松对继发性脊髓损伤的抑制作用 ,将 80只 SD大鼠随机分成 3组 ,用 Allen WD法致脊髓不完全性损伤。对照组 (A组 )只切除椎板 ,不损伤脊髓 ,不给药 ;模型组 (B组 )造模后应用生理盐水静脉注射 ;治疗组分成造模前 30 min给药组(C1 组 )及 30 min后给药组 (C2 组 )按 2 m g· kg- 1 的剂量静脉注射。于用药后 5 0 m in取伤段脊髓标本 ,纸上电泳法检测其兴奋性氨基酸含量 ;于造模后 2 4h取伤段脊髓标本 ,干湿重法测定水含量 ,原子吸收光谱法测定离子含量 ;于造模后 72 h取脊髓标本进行光镜及电镜观察。结果显示 ,同 A组比较 ,B组脊髓组织兴奋性氨基酸含量早期显著增高 (P<0 .0 5 ) ,水钠、钙含量也显著增高 (P<0 .0 1) ,钾、镁含量则显著减少 (P<0 .0 1) ,组织变性坏死程度严重。与 B组比较 ,C1 组脊髓组织兴奋性氨基酸含量显著降低 (P<0 .0 1) ,水钠、钙含量显著减少 (P<0 .0 1) ,钾镁含量显著增高 (P<0 .0 1) ,组织变性坏死程度较轻。同 B组比较 C2 组脊髓组织兴奋性氨基酸含量无显著性差异 (P>0 .0 5 ) ,然而其水钠、钙含量却显著降低 (P<0 .0 1) ,钾、镁含量显著增高 (P<0 .0 1) ,组织变性坏死程度较轻 ,但重于 C1 组。认为早期应用地塞米松可以有效地抑制继发性脊髓损伤。To explore the inhibition of Dexamethasone on the secondary spinal cord injuries. Methods: Eighty SD rats were randomly divided into 3 groups: Group A (control group: only made laminectomy without the injury of the spinal cord, not given medicine), Group B (model group: made laminectomy and the incomplete injury of the spinal cord, treated with 0.9% salt solution), Group C 1 (treatment group: given Dexamethasone at the time of 30 min before being made laminectomy and the incomplete injury of the spinal cord ), Group C 2 (treatment group: given Dexamethasone at the time of 30 min after being made laminectomy and the incomplete injury of the spinal cord ). Fifty min after medication, the samples of injured spinal cord were taken out to measure the content of Excitatory Amino Acid (EAA) with the method of paper electrophoresis; twenty two hours after modeling, the samples of injured spinal cord were taken out to measure the content of water and the content of ion with the method of dry and wet weight test, atomic absorption spectrophotometry respectively; seventy two hours after modeling, the samples of injured spinal cord were taken out to be observed through light and electron microscope. Results: ①Compared with Group A, the content of Excitatory Amino Acid (EAA) and the content of sodium, calcium were significantly increased, while the content of potassium, magnesium was decreased significantly, the degree of degeneration and necrosis was serious in Group B. ②Compared with Group B, the content of Excitatory Amino Acid (EAA) and the content of sodium, calcium were significantly decreased, while the content of potassium, magnesium was increased significantly, the degree of degeneration and necrosis was slight in Group C 1. ③Compared with Group B, the content of sodium, calcium was significantly decreased, while the content of potassium, magnesium was increased significantly, the degree of degeneration and necrosis was slight in Group C 2 (while was more serious than that in Group C 1 ), there was no di

关 键 词：地塞米松 继发性脊髓损伤 兴奋性氨基酸 离子 实验研究 动物 

分 类 号：R965[医药卫生—药理学]