胶原蛋白酶解物的降压作用及生物学特征  

The Blood Pressure Reduction Effect and Biological Properties of Collagen Enzymatic Hydrolysate

作　　者：张义军[1] 康白[1] 任维栋[2] 耿秀芳[2] 陈维宁[1] 张薇[1] 

机构地区：[1]潍坊医学院药理学教研室,山东潍坊261042 [2]潍坊医学院生物化学教研室,山东潍坊261042

出　　处：《潍坊医学院学报》2001年第4期275-277,W002,共4页Acta Academiae Medicinae Weifang

基　　金：潍坊市科委资助课题 (课题号 :1 999 0 2 1 )

摘　　要：目的　研究胶原蛋白酶解物 (CEH)对大鼠血压、豚鼠肠平滑肌及猪肺ACE的作用。方法　选用正常麻醉大鼠、SHR及肾性高血压大鼠 ,采用颈动脉插管法检测CEH对血压的影响 ;CEH对离体豚鼠回肠平滑肌的作用。结果　CEH对猪肺血管紧张素转换酶 (ACE)的IC50 为 2 6μmol/L。 1.5g/kg静脉注射能降低正常麻醉大鼠和SHR大鼠的血压 ,1.0g/kg静脉注射对肾性高血压大鼠也引起降压效应。单次口服 2g/kgCEH ,正常及SHR大鼠血压无明显变化 ,连续应用 ( 2g/kg) 15d ,SHR大鼠则呈现弱的降压效应。 7.5g/lCEH对离体豚鼠回肠平滑肌产生兴奋收缩效应。Objective The effects of collagen enzymatic hydrolysate (CEH) on blood pressure in rat,ileal smooth muscle of guinea pig and angiotensin converting enzyme (ACE) of pig lung were studied in this paper.Method The blood pressue were measured in nomal anesthetized rat and SHR and renal hypertension rats by arterria carotis communis method.Result The IC 50 of CEH on angiotensin converting emzyme of pig lung was 26μmol/Lmol/L.1.5g/kg,iv.CEH could reduce blood pressure of normal anesthetized rats and SHR rats and could also reduce the blood pressure of renal hypertension rats at the dose of 10g/kg,iv.Single dose application of CEH had no significant effect on normal and SHR rats' blood pressure,while continuous application of CEH at the dose of 2g/kg had mild blood pressure reduction effect on SHR rats' blood pressure.Excitation contraction effect on isolated ileal smooth muscle of guinea pig occurs at the concentration of 7.5 mg/ml.Conclusion CEH has the effect of blood pressure reduction,and smooth muscle contraction.This effect may be related to inhibit ACE.

关 键 词：胶原蛋白酶解物 血压 血管紧张素转换酶 高血压 

分 类 号：R544.105[医药卫生—心血管疾病]