复方丹参滴丸中冰片的生物体液的含量测定  

GC-MS Assay for Borneol of Compound Danshen Dropping Pill in Serum

作　　者：黄天来[1] 王宁生[1] 

机构地区：[1]广州中医药大学临床药理研究所,广州510405

出　　处：《中药新药与临床药理》2001年第5期354-358,共5页Traditional Chinese Drug Research and Clinical Pharmacology

基　　金：国家自然基金课题(39770909)

摘　　要：以ＧＣ—ＭＳ联用技术测定大鼠灌服复方丹参滴丸后其血清中的冰片合量。冰片浓度在０．００５０５～４０．１μ／ｍｌ范围内线性关系良好。血中回收率达７２．８２～８６．７７％，最低检测浓度为１ｎｇ／ｍＬ。本方法以萘为内标，ＳＩＭ方式检测，定量准确、干扰少，日内及日间的ＲＳＤ小于２．７６％和１．９３％。结果表明本方法可应用于复方丹参滴丸所含冰片的药代动力学等的进一步的研究。A specific and sensitive GC - MS method for assaying Borneol in the serum of rats medicated orally with compound Danshen dropping pill was developed. Naphthalene was used as the internal standard, the dectection was performed by SIM. The calibration curve was linear in the range from 0.00505 to 40. 1 μg/mL, the detectable limit of this method was 1 ng/mL. The extraction recovery of Borneol was 72.82% - 86.77%. The RSD of within - day and day - to - day was less than 2.76% and 1.93% respectively. The results showed that this method was simple, sensitive, accurate and good reproducibility , and can be used in further pharmacokinetic study of Borneol in danshen Dropping pill.

关 键 词：复方丹参滴丸 冰片 GC-MS 中药 含量 测定 

分 类 号：R285.6[医药卫生—中药学]