中华绒螯蟹与日本绒螯蟹线粒体COI基因片段的序列比较研究  

Comparative Study of Mitochondrial COI Gene Fragment Between Chinese Mitten Crab (Eriocheir sinensis)and Japanese Mitten Crab (Eriocheir japonica)

作　　者：孔晓瑜[1] 喻子牛[1] 刘亚军[1] 高天翔[1] 武云飞[1] 

机构地区：[1]青岛海洋大学水产学院教育部水产养殖研究重点实验室,青岛266003

出　　处：《青岛海洋大学学报（自然科学版）》2001年第6期861-866,共6页Journal of Ocean University of Qingdao

基　　金：高等学校骨干教师资助计划项目;留学回国人员科研启动基金资助

摘　　要：以相应引物 PCR扩增了黄河口中华绒螯蟹线粒体细胞色素氧化酶 I亚基基因 (COI)片段 ,PCR产物经 T载体连接之后进行克隆、测序 ,得到 70 9bp的碱基序列 ,其 A,T,G,C含量分别为 34.4 1% ,2 7.93% ,2 0 .0 3%和 17.6 3%。并比较它与珠江流域中华绒螯蟹 COI序列和日本绒螯蟹 COI序列的差异 ,发现黄河口中华绒螯蟹与珠江流域中华绒螯蟹 COI序列完全相同 ,而与日本绒螯蟹差异非常明显 ,70 9或 6 5 8(不计引物 )位点中核苷酸差异数为 32 ,核苷酸差异率为 4 .5 1%或 4 .86 % (不计引物 ) ,其中 2 5个位点为转换 ,7个位点为颠换。作者倾向于支持存在中华绒螯蟹和日本绒螯蟹 。Mitochondrial COI gene fragment of Chinese mitten crab ( Eriocheir sinensis ) from Yellow River was amplified via PCR, the PCR products were ligated into T vector, cloned and sequenced. 709 bp nucleotide sequences were retrieved. The content of A, T, G and C in the fragment were 34.41%, 27.93%, 20.03% and 17.63% respectively. The sequence comparison was made among Chinese mitten crab from Yellow River and from Zhujiang River and Japanese mitten crab ( E. japonica ). Chinese mitten crabs from Yellow River and from Zhujiang River had the same nucleotide sequence, but they differed significantly from Japanese one; nucleotide difference number between the two species was 32, in which 25 were transitions and 7 were transversions. The result tended to support the opinion that Chinese mitten crab and Japanese mitten crab could be two species or different geographicsubspecies of the same species.

关 键 词：中华绒螯蟹 日本绒螯蟹 细胞色素氧化酶Ⅰ亚基基因 序列测定 碱基序列 

分 类 号：Q959.223[生物学—动物学] Q953