高效液相色谱法测定皮疹清颗粒中黄芩苷的含量  

Determination of baicalin in Pizhenqing granules with HPLC

作　　者：芦金清[1] 余南才[2] 李竣[1] 胡克菲[2] 赵享法[1] 

机构地区：[1]湖北中医学院药学系,湖北武汉430061 [2]武汉市第一医院,湖北武汉430022

出　　处：《中国医院药学杂志》2001年第11期661-663,共3页Chinese Journal of Hospital Pharmacy

摘　　要：目的 :建立用高效液相色谱法测定皮疹清颗粒中黄芩苷含量的的方法。方法 :采用NucleosilC18,流动相为乙腈 1%醋酸 (2 6∶74) ,流速为 1.2ml·min-1,紫外检测波长为 2 76nm。结果 :黄芩苷浓度线性范围为 15～ 41μg ,r为0 .9994,平均回收率为 99.16 % ,RSD为 0 .92 % (n =6 )。结论 :本法操作简便 ,准确 ,重复性好 。OBJECTIVE To establish a method for determining the content of baicalin in Pizhenqing granules by HPLC.METHODS A nucleosil C 18 column with acetonitrile 1% acetic acid solution (26∶74) as the mobile phase was used.The flow rate was 1.2 ml·min -1 and the detective wavelength was 276 nm.RESULTS The calibration curve was linear at the range of 15～41 μg for baicalin ( r = 0.9994 ).The average recovery was 99.16% and RSD was 0.92% ( n =6).CONCLUSIONS The method is simple,and accurate and can be used as quantitative method for this preparation.

关 键 词：皮疹清颗粒 黄芩苷 含量 测定 高效液相色谱法 中药 HPLC 

分 类 号：R286.0[医药卫生—中药学]