首例中国株乙型肝炎病毒D基因型全序列测定及分析　  

Determination and analysis of complete nucleotide sequence of Chinese genotype D hepatitis B virus: the first report

作　　者：阎丽[1] 侯金林[1] 郭亚兵[1] 陈金军[1] 王战会[1] 林裕龙[1] 骆抗先[1] 牛贞玉 

机构地区：[1]第一军医大学南方医院感染内科,广东广州510515 [2]兰州军区解放军第五陆军医院,宁夏银川750004

出　　处：《第一军医大学学报》2001年第11期848-851,共4页Journal of First Military Medical University

基　　金：国家自然科学基金(39630280);广东省自然科学基金(980223)

摘　　要：目的　测定并分析１例中国慢性无症状携带者感染的乙型肝炎病毒（ＨＢＶ）Ｄ基因型的全基因序列。方法　用聚合 酶链式反应（ＰＣＲ）扩增ＨＢＶ全基因，将ＰＣＲ产物克隆后对其进行核酸序列分析并与已发表的ＨＢＶ毒株全序列进行 同源性比较；对 ＧｅｎＢａｎｋ中已发表的３０株ＨＢＶ Ｄ基因型毒株的全序列进行系统进化树分析。结果　该病毒全基因长 ３１８２ｂｐ，为ａｙｗ亚型，Ｄ基因型；此毒株全基因ＧｅｎＢａｎｋ　ＡｃｃｅｓｓｉｏｎＮｏ为ＡＦ２８０８１７；与ＧｅｎＢａｎｋ中已发表的ＨＢＶＤ 基因型全序列同源性为９８．３％～９４．5％，与已发表的 Ａ、Ｂ、Ｃ、Ｅ、Ｆ和Ｇ基因型全序列同源性均小于８９．５％。结论　首例中 国株 ＨＢＶ Ｄ 基因型全序列与源于瑞典的四株 ＨＢＶ Ｄ 基因型全基因的进化距离最近。Objective To determine the complete nucleotide sequence of a Chinese hepatitis B virus (HBV) strain of genotype D. Method The complete nucleotide sequence of HBV derived from a Chinese chronic asymptomatic carrier was amplified by PCR and cloned to conduct sequence analysis. Homology of the resulted nucleotide sequence with those of published HBV strains was assessed by using DNASIS, and complete sequence analysis of the phylogenetic tree of 30 genotype D HBV strains conducted by the assistance of Clustaw. Results With the complete nucleotide sequence of 3 182 bp, this HBV strain belongs to ayw3 subtype and D genotype, with the Genebank accession number AP2808 17. Its complete nucleotide homology is 98.3% to 94.5% with the published sequences of genotype D HBV strain and lea than 89.5% with the other HBV strains of genotype A, B, C, E, F and G published in GenBank. Conclusion The evolutionary relations of the complete nucleotide sequence of this HBV strain is the closest to those ofthe4 Swedish strains out of the 30 genotype D strains published in GenBank.

关 键 词：乙型肝炎病毒 D基因型 序列分析 进化树 HBV 

分 类 号：R373.21[医药卫生—病原生物学]