显微注射技术制备转基因豚鼠早期胚胎的初步探讨  

Production of Early Transgenic Guinea-pig Embryos by Microinjection

作　　者：夏仁品[1] 陈耿臻[1] 文军[1] 万云乐[1] 沈文律[1] 蒋学武[1] T.Uchida 

机构地区：[1]汕头大学医学院第二附属医院普外科,广东汕头515041 [2]日本SapporoHokuyu医院

出　　处：《汕头大学医学院学报》2001年第3期180-181,共2页Journal of Shantou University Medical College

基　　金：广东省自然科学基金资助课题 (编号 :970 6 10 );日本SapporoHokuyu医院科研基金资助

摘　　要：目的 :应用显微注射方法生成转基因豚鼠。方法 :将外源DNA注射到用人工注射性激素超排卵诱导的豚鼠受精卵内 ,体外培养数小时后移植到假孕母鼠输卵管中使其体内发育 ,发育到第 7天处死假孕母鼠 ,回收早期胚胎观察其存活情况。结果 :显微注射成功率达 85 % ;早期胚胎存活率低 (15个中仅 2个存活 )。结论 :显微注射技术是一种简便、可重复性的好方法。Objective: To generate transgenic Guinea-pig by microinjection. Methods: Guinea-pig pretreated with a dose of 30u of pregnant mare serum gonadotropin(PMSG) containing follicle stimulating hormone(FSH), then with the same dose of human chorionic gonadotropin(HCG) at 24 hours after injection of PMSG, and was killed at 15～17 hours after this treatment. Fertilized egg was obtained from this dead super ovulated females, and then received the microinjection of target DNA at its pronucleus, followed by transferring into the oviduct of surrogate mothers on day of their pseudopregnancy, surrogate mothers were killed to obtain early transgenic-Guinea-pig embryos after 7 days, and the early embryos were examined by microscope. Results: Eggs with successful microinjection of target DNA accounted for 85%, out of 15 eggs of early transgenic Guinea-pig embryos only 2 eggs survived. Conclusion: Microinjection is available, and good method for transferred gene into cells. A routine production of transgenic Guinea-pig with microinjection needs further study.

关 键 词：转基因豚鼠 显微注射 早期胚胎 DNA 器官移植 

分 类 号：R617[医药卫生—外科学] Q132.7[医药卫生—临床医学]