慢性乙型肝炎患者HBcAg特异性Th细胞克隆的建立及初步分析  

Establishment and analysis of HBcAg-specific T helper clones in patients with chronic hepatitis B

作　　者：姜荣龙[1] 冯筱榕[1] 郭亚兵[1] 卢桥生[1] 侯金林[1] 章廉[1] 骆抗先[1] 富宁[2] 

机构地区：[1]第一军医大学南方医院感染内科,广东广州510515 [2]第一军医大学免疫学教研室,广东广州510515

出　　处：《第一军医大学学报》2001年第9期676-678,共3页Journal of First Military Medical University

基　　金：国家自然科学基金(39630280)

摘　　要：目的 了解Th细胞极化群体在慢性乙型肝炎（CHB）外周血及肝组织中的分布情况。方法 采用有限稀释法，建立CHB患者外周血及肝组织HBcAg特异性Th细胞克隆，并对所获得细胞克隆进行分析。结果 发现自肝组织分离的HBcAg特异性T细胞克隆，92.5%为CD4+表型，以Th1为主；而来源于外周血的T细胞克隆主要以Th0 为主，并与HBeAg是否阳性无关。仅15% （6/40）的T细胞克隆呈现明显的HBcAg特异性淋巴细胞增殖反应。结论 在肝组织及外周血占主导地位的Th细胞极化群的不同，较高比例的Th1细胞在肝组织炎症局部的聚集，提示在HBV的免疫发病机制中，Th1细胞可能起重要作用。 Objective To investigate the distribution of T helper cell polarized populations in patients with chronic hepatitis B (CHB). Method Freshly isolated mononuclear cells in the liver and peripheral blood of CHB patients were obtained, and HBcAg-specific T helper cell clones were subsequently established by limiting dilution, and the analysis of these clones was conducted. Results The majority of liver-infiltrating T cells in CHB patients were Th1 cells capable of secreting IFN-γ but not IL-4. The clones derived from the peripheral blood were mostly Th0 cells, irrespective of the HBeAg status of the patient. Only a fraction of 15% of the T cell clones presented obvious HBcAg-specific proliferation response. Conclusion The varied predominance exercised by different polarized T cell populations in the liver and peripheral blood, and the aggregation of Th1 cells at a large proportion to other T helper cell populations in the inflammation locality, indicate that Th1 cells may play an important role in the immune pathogenesis of CHB.

关 键 词：慢性乙型肝炎 Th细胞克隆 免疫病理 HBCAG 

分 类 号：R512.62[医药卫生—内科学]