父儿传播乙型肝炎病毒S区的基因分型  

THE GENETIC CLASSIFICATION BY S GENE OF HBV IN TRANSMISSION FROM FATHER TO FETUS.

作　　者：彭桂福[1] 王珊珊[1] 姜普林[1] 曾年华[1] 王志斌[1] 

机构地区：[1]广州军区军事医学研究所,广州510507

出　　处：《现代预防医学》2001年第4期413-415,共3页Modern Preventive Medicine

摘　　要：目的 :从乙型肝炎病毒 (HBV) S基因 15 5～ 6 87段序列 ,研究携带者父亲与其子所携 HBV的基因分型。方法 :应用套式 PCR扩增 S基因片段 ,直接测序 15 5～ 6 87段核苷酸并推衍出其编码的氨基酸序列 ,与文献中 4株分属 A、 B、C、 D基因序列比较。结果 :父亲与其子所携 HBV序列与 B型同源性最高 ,核苷酸水平分别达 98.3%与 97.9%,氨基酸水平分别达 10 0 %与 99.4%,故父亲与其子所携 HBV均属 B型。父与子间此段序列的同源性极高 ,核苷酸与氨基酸同源性分别达 99.6 %与 99.4%。结论 :从分子水平证明出生前期 HBV父儿传播的存在。Objective:To study HBV genetic classification of transmission from father to fetus.Methods:The S gene fragment of HBV was amplified by nested polymerase chain reaction and sequenced.Results:The S gene 155~687 nucleotide sequence was acquired and the corresponding amino acid sequence was deduced.Compared to previously described HBV genotypes via A,B,C,D,they were most similar to the B genotype.The homology of nucleotide respectively were 98.3% and 97.9%,100% and 99.4% homology at amino acid level.So HBV of father and fetus were inferred B genotype.The HBV homology of the father and fetus was 99.6% at nucleotide,99.4% at amino acid level.Conclusion:HBV transmission from father to fetus was existence.

关 键 词：乙型肝炎病毒 父婴传播 基因分型 乙型肝炎 

分 类 号：R512.620.1[医药卫生—内科学]