邻苯三酚自氧化法测定血清超氧化物歧化酶变异因素的探讨和控制  

Study of variance control in assay of serum superoxide dismutase by pyrogallol autoxidation method

作　　者：张建新[1] 石南宁[1] 康学军[1] 王彦[1] 黄新生[1] 

机构地区：[1]东南大学公共卫生学院,江苏南京210009

出　　处：《东南大学学报（医学版）》2001年第3期146-149,共4页Journal of Southeast University(Medical Science Edition)

基　　金：铁道部科技基金资助项目 (J99Z1 37)

摘　　要：目的 :研究邻苯三酚法测定血清超氧化物歧化酶 (SOD)的变异因素。方法 :在pH 8.30KH2 PO4 Na2 B4O7缓冲溶液中 ,用维生素C溶液中止邻苯三酚自氧化反应的固定时间法 ,研究反应温度波动± 1℃、EDTA用量和维生素C对SOD活性测定的影响。结果 :反应温度在 2 5～ 37℃范围内 ,温度波动± 1℃ ,预测SOD活性测定的相对偏差为 5 %～ 16% ,与实验结果一致 ;反应液中EDTA浓度≥ 0 .13mmol·L-1、样品中维生素C浓度≥ 0 .12mmol·L-1时 ,SOD的测定结果产生大的偏性。结论 :邻苯三酚法测定血清SOD反应温度最好控制在± 0 .Objective:To study the variance factors in assay of serum superoxide dismutase by pyrogallol autoxidation method.Methods:The pyrogallol autoxidation in pH 8.30 KH 2PO 4 Na 2B 4O 7 buffer solution was ended by adding 40?g·L -1 vitamin C reagent at the fixed time.Effects of the fluctuation of reaction temperature,the quantity of EDTA and vitamin C were investigated.Results:The fluctuation of ±1?℃ of the reaction temperature was obtained from 25?℃ to 37?℃,the relative deviations of superoxide dismutase activity was 5%～16% with a good agreement to the estimated values.The quantity of EDTA(≥0.13?mmol·L -1 in reaction solution)and vitamin C(≥0.12?mmol·L -1 in serum)are bias factors in assay of the superoxide dismutase activity by pyrogallol autoxidation method.Conclusion:The fluctuation of the reaction temperature had better be controlled within ± 0.2 ?℃.

关 键 词：邻苯三酚法 超氧化物歧化酶 变异控制 血清 测定 

分 类 号：R446.112[医药卫生—诊断学]