一氧化氮对肿瘤坏死因子-α诱导的大鼠血管平滑肌细胞细胞间粘附分子-1表达的影响  

Influence of NO on Expression of Intercellular Adhesion Molecule-1 in Rat Vascular Smooth Muscle Cells

作　　者：张梅[1] 温进坤[1] 韩梅[1] 

机构地区：[1]河北医科大学基础医学研究所生物化学研究室,河北省石家庄市050017

出　　处：《中国动脉硬化杂志》2001年第5期373-375,共3页Chinese Journal of Arteriosclerosis

基　　金：国家自然科学基金 ( 39970 2 74);河北省自然科学基金( 30 135 8)

摘　　要：为探讨肿瘤坏死因子 α介导单核细胞与血管平滑肌细胞粘附致动脉粥样硬化及一氧化氮抗动脉粥样硬化的机制 ,用体外培养的大鼠血管平滑肌细胞 ,以硝普钠为一氧化氮供体 ,采用细胞粘附实验、流式细胞术和逆转录—聚合酶链反应观察一氧化氮对肿瘤坏死因子 α诱导的细胞间粘附分子 1在血管平滑肌细胞中的表达及其对单核细胞与血管平滑肌细胞粘附的影响。结果发现一氧化氮显著抑制肿瘤坏死因子 α诱导的单核细胞与血管平滑肌细胞的粘附及细胞间粘附分子 1在血管平滑肌细胞中的表达 ,减少血管平滑肌细胞表面的细胞间粘附分子 1。结果表明一氧化氮抑制细胞间粘附分子 1基因表达及降低单核细胞与血管平滑肌细胞之间的粘附率是其抗动脉粥样硬化的机制之一。Aim To investigate the influence of NO on expression of intercellular adhesion molecule-1(ICAM-1) in vascular smooth muscle cells (VSMC) and adhesion between monocyte and VSMC induced by TNF-α. Methods Cell adhesion, flow cytometry and RT-PCR were applied to study effects of nitroprusside(SNP) and NO, on adhesion between monocyte and VSMC and expression of ICAM-1 gene. Results Adhesion between monocyte and VSMC was significantly inhibited by SNP. Expression of ICAM-1 gene in VSMC induced by TNF-α was depressed after VSMC was treated with SNP, and level of ICAM-1 on VSMC was reduced. Conclusions It is one of the mechanisms of anti-atherosclerosis by NO that expression of ICAM-1 gene in VSMC and adhesion between monocyte and VSMC induced by TNF-α could be inhibited by NO.

关 键 词：一氧化氮 细胞间粘附分子-1 单核细胞 动脉硬化 血管平滑肌 

分 类 号：R543.502[医药卫生—心血管疾病]