鸡贫血病病毒长春株VP3基因的克隆及序列分析  

Cloning and Sequence Analysis of VP3 Gene of Chicken Anemia Virus Changchun Isolate

作　　者：宋秀龙[1] 章金刚[2] 常国权[2] 陈奖励[1] 

机构地区：[1]中国农业科学院哈尔滨兽医研究所兽医生物技术国家重点实验室,黑龙江哈尔滨150001 [2]解放军军需大学动物科技系,吉林长春130062

出　　处：《中国兽医学报》2001年第6期546-548,共3页Chinese Journal of Veterinary Science

基　　金：农业部"九五"重点课题 ( 95牧 0 1-0 3-0 3)

摘　　要：采用 PCR扩增方法成功克隆了鸡贫血病病毒 (CAV)长春分离株的 VP3基因 ,并进行了核苷酸序列测定。通过与 TK5 80 3、SJ1株进行序列比较 ,发现长春分离株 VP3基因与 TK5 80 3仅有 1个碱基差异 ,而氨基酸序列完全相同 ;与山东 SJ1株有 3个碱基差异和According to the nucleotide sequence in NCBIG GeneBank, the VP3 gene of a chicken anemia virus isolated from changchun was amplified by PCR, and cloned to the SmaⅠ site of pUC119 plasmid. The nucleotide sequence was analysed. By comparison with TK5803 and SJ1, the VP3 gene of Changchun isolate was demonstrated to have only one nucleotide and no amino-acid different from TK5803, three nucleotides and three amino-acids different from SJ1.

关 键 词：鸡 贫血病病毒 VP3基因 基因克隆 序列分布 

分 类 号：S858.31[农业科学—临床兽医学] S852.65[农业科学—兽医学]