克隆伊氏锥虫对安锥赛抗药性的培育  

Development of Resistance of Trypanosoma evansi to Antrycide

作　　者：廖党金[1] 沈杰[1] 周勇志[1] 

机构地区：[1]中国农业科学院上海家畜寄生虫研究所,上海200232

出　　处：《中国兽医学报》2001年第6期562-564,共3页Chinese Journal of Veterinary Science

摘　　要：为了深入观察伊氏锥虫对安锥赛抗药性的形成 ,将伊氏锥虫浙江水牛株克隆 ,克隆繁殖后的虫体一部分作为原种对照组 ,即 C0 1 组 ;一部分虫体连续经环磷酰胺免疫抑制的小鼠 ,再用安锥赛亚治疗剂量治疗该小鼠 ,使其伊氏锥虫对安锥赛产生 7个不同程度的抗药性组 ,即 C1 ～ C7组 ;另一部分虫体也连续经环磷酰胺免疫抑制的小鼠 ,作为伴随 C7的同步繁殖组 ,即不用药物的 C0 2 组。结果 C0 1 、C0 2 、C1 ～ C7组所用安锥赛剂量依次为 0、0、6、16、40、80、196、40 6、6 38mg/kg,它们经小鼠传代数依次为 0、2 8、1、3、6、9、15、2 2、2 8代。用体外生长繁殖抑制法测得它们的 IC50 值依次为0 .0 15 5 0、0 .0 16 87、0 .0 82 6 0、0 .10 2 37、1.40 92 9、1.92 2 90、9.92 330、2 3.5 6 30 0、43.840 0 0。结果表明 ,安锥赛亚治疗剂量与伊氏锥虫抗药性的 IC50 值呈曲线关系 ,从 C1 到 C4的 IC50 值上升较缓慢 ,从 C4后的 IC50 值上升较快 ,并且几乎呈直线上升 ;伊氏锥虫所经小鼠的代数与它们的 IC50 值的关系呈抛物线型 ,从 C1 到 C4的 IC50 值上升很慢 ,C4以后的 IC50 值上升很快。用体内试验测试 C0 1 ～C4组的 CD1 0 0 值 ,每个组感染 35只小鼠 ,每只小鼠接种 1.0× 10 4条锥虫 ,其中A clone of T.evansi,C 01,from Zhejiang buffalo strain was sensitable to antrycide Trypanosomes from C 01 developed seven levels of resistance to antrycide,C 1-C 7,through 1,3,6,9,15,22 and 28 generation of immunosuppressed mice(cyclophosphamide) treated with antrycide at sub-dose by i.p.Another trypanosomas from C 01 were as C 02 through 28 generation of immunosuppressed mice treated without drug.The inhibiting growth assay was used to determine their sensitivities to antrycide.The IC 50 of the C 01,C 02,C 1,C 2,C 3,C 4,C 5,C 6 and C 7 was 0.015 50, 0.016 87, 0.082 60, 0.102 37, 1.409 29, 1.922 90, 9.923 30, 23.563 00 and 43.840 00 in turn.The results showed that the relationship between used total quantities of antrycide and IC 50 was a curve pattern,meaning slow rise of the resistance to antrycide from C 1 to C 4 and then fast rise of it from C 4 to C 7.On the other hand,the relationship between the quantities of used immunosuppressed mice and the IC 50 was a parabola pattern.The treated mouse test was used to determine CD 100 of C 01,C 1,C 2,C 3 and C 4.The CD 100 of C 01 and C 1 were measured to be 7.0 mg/kg and 13.0 mg/kg,respectively.The CD 100 of the other 3 groups were not obtained because of their heavy resistance to antrycide.These results indicated that the resistance of cloned T.evansi to the drug could develop rapidly through immunosuppressed mice.

关 键 词：伊氏锥虫 克隆 安锥赛 抗药性 小鼠 

分 类 号：S852.7[农业科学—基础兽医学]