中华鳖心组织成纤维细胞系的建立及特性  

Establishment and Characteristics of Heart Fibroblast Cell Line of the Soft-shell Turtle(Trionyx sinesis)

作　　者：李正秋[1] 张奇亚[1] 

机构地区：[1]中国科学院水生生物研究所淡水生态与生物技术国家重点实验室,湖北武汉430072

出　　处：《中国兽医学报》2001年第6期583-585,共3页Chinese Journal of Veterinary Science

基　　金：国家"973"计划资助项目 ( G19990 5 390 8);中国科学院生物科学与技术研究特别资助项目 ( STZ-0 0 -13)

摘　　要：以中华鳖心组织为材料 ,用含 10 %胎牛血清的 TC199培养基 ,于 2 5℃条件下 ,经 14个月传代培养 6 0余次 ,获得一个能稳定生长、以成纤维样细胞为主的细胞系 ,称之为 TSH(Trionyx sinesis heart)细胞系。分别对其原代和传代细胞的染色体数目、不同温度下细胞的生长曲线及原代和传代细胞的显微形态进行了测定和比较。结果表明 ,细胞在2 5℃条件下生长速度最快 ;原代细胞约有 6 4%的染色体为 2 n=6 6 ,而传代细胞为四倍体的有 6 2 %。初步估计的细胞周期为 2～ 3d。传代细胞冻存后 ,复苏状态良好 。A cell line of soft-shell turtle from heart tissues has been successfully established.The primary cell culture and subculture are by TC199 medium containing 10% calf serum and incubated at temperature about 25℃,and through more than 60 subculture passages over 14 months.It is a stable grown fibroblast-like cell line,which is named TSH(Trionyx sinesis heart) cell line.Some biological characteristics of the cell line are described briefly.The chromosome numbers of primary culture and subculture passage,the growing curve of subculture passage at different temperature 15℃,20℃ and 25℃,and the morphological of TSH cell line of primary and subculture are detected and compared respectively.The cells grow fast at 25℃.The primary cells that had a normal number of chromosomes(2n=66) accounted about 64%;but the chromosomes of cell line were determined as a quadrupled and accounted about 62%.The cell cycle time of TSH is between 2-3 d.The rejuvenation of the subculture passage cells was tested to be well.

关 键 词：中华鳖 心组织 成纤维细胞 细胞系 组织培养 

分 类 号：S947.1[农业科学—水产养殖]