西比灵对脑缺血再灌注时细胞凋亡及病理形态改变的影响  

The effect of FNZ on cell withering and the pathomorphological changes in cerebral ischemic reperfusion

作　　者：张秋玲[1] 邢国庆[1] 邱玉芳[1] 李亚鲁[1] 王新成[1] 孟庆华[2] 

机构地区：[1]泰山医学院,山东泰安271000 [2]泰山慢性病医院,山东泰安271000

出　　处：《泰山医学院学报》2001年第3期187-189,共3页Journal of Taishan Medical College

摘　　要：目的　研究预防性应用西比灵对脑缺血再灌注时细胞凋亡及病理形态改变影响的机理。方法　取沙土鼠 2 1只 ,随机分为三组 ,(1)假手术组 (2 )缺血再灌注组 (3)西比灵处理组 ,采用双侧颈总动脉夹闭法复制沙土鼠脑缺血再灌注模型 ,于术后 3天处死动物取材 ,石蜡切片 ,光镜观察 ,超薄切片 ,电镜观察 ,TUNEL法显示凋亡细胞 ,观察CA1区神经细胞病理形态的改变及细胞凋亡情况 ,结果　缺血再灌注组可见CA1区细胞呈现缺血性改变 ,预防性应用西比灵可明显减轻海马CA1区神经细胞缺血性损伤并减少细胞凋亡的表达 ,统计学处理后 ,缺血组与西比灵处理组凋亡细胞数有明显差异 ,P <0 0 5 。Objective: To research into the mechanism of the effect on cell withering and the pathomorphological changes induced by preventive application of FNZ in cerebral ischemic reperfusion. Methods: Twenty one pallasiomies were randomly divided into the three groups: sham operation group, reperfusion group and FNZ treated group. The pallasiomies' cerebral ischemic reperfusion models were made by the bilateral occlusion of common carotid arteries. The parrafin sections from the experimental hippocampil killed 3 daysafter the operation were observed by light microscope and the ultrathin sections by electron microscope. Withered cells were shown by the method of TUNEL, and the pathomorphological changes of the neurons and the withered cells in CA1 were observed. Results: The ischemic reperfusion group showed obvious ischemic changes of the cells in CA1 and the preventive application of FNZ obviously reduced the cell lesions in CA1. Processed statistical data showed obvious differences (P<0 05) between the ischemic group and the FNZ group in withered cells. Conclusion: FNZ can reduce the cerebral cell lesions and cell withering in ischemic reperfusion.

关 键 词：西比灵 细胞凋亡 脑缺血 再灌注损伤 

分 类 号：R965[医药卫生—药理学]