克隆的丙型肝炎病毒NS2基因中发现数个N端终止突变序列  

Discovery of Several N-terminal Stop Codons in NS2 Region of Hepatitis C Virus

作　　者：冷彦[1] 陈秀珠 叶嗣颖[1] 杜勇[2] 杜桂鑫[2] 王海涛[2] 

机构地区：[1]华中科技大学同济医学院基础医学院微生物学教研室,武汉430030 [2]军事医学科学院微生物流行病研究所,北京100071

出　　处：《同济医科大学学报》2001年第5期401-403,共3页Acta Universitatis Medicinae Tongji

基　　金：国家自然科学基金重点资助项目 ( No.3 963 0 0 2 0)

摘　　要：探讨丙型肝炎病毒 ( HCV)准种在 NS2区的基因结构特征。利用逆转录 -巢式 PCR从一份 HCV慢性携带者的阳性血清及一份急性丙肝患者的血清中获得 HCV NS2全长 c DNA,将其克隆于 T载体 ,各随机挑取 5个阳性克隆进行序列测定。结果显示在克隆到的 HCV NS2全长基因中 ,慢性携带者该区序列有一个于 HCV多聚蛋白第 83 5位氨基酸的位置出现终止信号 ,丙肝患者该区序列有三个于第 83 5位氨基酸的位置出现终止信号 ,一个小于第 887位氨基酸的位置出现终止信号。在克隆到的 HCV NS2基因中存在多个 N端终止突变序列 ,提示 NS2To explore the genetic variation characteristics of nonstructural (NS)2 quasispecies of hepatitis C virus, NS2 genes of HCV from a HCV asymptomatic carrier and a patient with abnormal ALT level were amplified by RT-nested-PCR and cloned into T vector. Five positive clones from each of them were selected randomly for sequencing. The results showed that one of the NS2 gene clones from the asymptomatic carrier had an in-frame stop codon at 835aa of HCV polyprotein. When it came to the patient with abnormal ALT level, 3 clones had stop codons at 835aa and 1 clone had a stop codon at 887aa of HCV polyprotein because of variation. Several N terminal stop codons in NS2 region of hepatitis C virus suggested that N-terminus of NS2 had close relation to envelop protein regions.

关 键 词：丙型肝炎病毒 NS2 基因结构 N端终止 基因突变序列 

分 类 号：R512.6[医药卫生—内科学] R373[医药卫生—临床医学]