CP基因转化的线辣椒抗卡那霉素和抗CMV特性的遗传  

Inheritance of kanamycin-resistance and CMV-resistance in transgenic chili pepper expressing CMV-CP and TMV-CP genes

作　　者：商鸿生[1] 王旭[1] 徐秉良[1] 

机构地区：[1]西北农林科技大学植保学院,陕西杨陵712100

出　　处：《西北农林科技大学学报（自然科学版）》2001年第5期103-106,共4页Journal of Northwest A&F University(Natural Science Edition)

基　　金：国家自然科学基金资助项目 ( 396 70 488) ;陕西省科技攻关项目 ( 96 K0 6 -G1-0 3)

摘　　要：利用根癌土壤杆菌 ,采用叶盘法转化线辣椒 (Capsicum annuum var.longunt)优良品种陕 82 12 ,建成了能同时表达 CMV和 TMV外壳蛋白基因 (CP基因 )的 T1 代纯合系。以其自交 T2 ～ T4代 ,以及 T2 代植株与未转化陕 82 12杂交的 F1 代和 F2 代群体为试材 ,研究了抗卡那霉素和抗黄瓜花叶病毒 (CMV)特性的遗传传递规律。结果发现 ,抗卡那霉素标记基因和抗病性基因在自交和杂交各代都能稳定地高效表达 ;两者在自交各代纯合 ;在杂交 F1 代抗药性和抗病性都表现为显性 ;在杂交 F2 代 ,抗药植株对敏感植株 ,以及抗病植株对感病植株的分离比都符合 3∶ 1的理论比例。Both CMV CP and TMV CP genes were transferred into cultivar 'Shaan 8212' of chili pepper( Capsicum annuum var. longunt )mediated by Agrobacterium tumefacience.Selfing generation T 2,T 3 and T 4 from homozygous T 1 lines and F 1,F 2 of cross T 2/Shaan 8212 were tested and analysed to reveal inheritance of resistance to Kanamycin or to CMV.The results showed that resistance steadily and strongly expressed in tested populations.T 2,T 3 and T 4 were in homozygous condition showing high resistance to Kanamycin and to CMV.The resistance was dominant over sensitivity to Kanamycin or susceptibility to CMV in F 1 progenies.F 2 segregated to fit 3∶1 ratio.It was concluded that resistance to Kanamycin and resistance to CMV were conditioned by single dominant genes.

关 键 词：转基因线辣椒 CP基因 CMV 卡那霉素 抗病性 遗传规律 育种 

分 类 号：S641.3[农业科学—蔬菜学]