长链烷基化物与牛血清白蛋白相互作用的共振光散射研究  

A STUDY ON THE INTERACTION OF LONG CHAIN ALKYLCOMPLEX WITH BOVINE SERUM ALBUMIN AS MEASURED BYRESONANCE LIGNHT SCATTERING

作　　者：奉萍[1] 黄承志[1] 舒为群[2] 

机构地区：[1]西南师范大学环境化学研究所 [2]第三军医大学预防医学系,重庆400038

出　　处：《环境化学》2001年第5期497-502,共6页Environmental Chemistry

基　　金：国家自然科学基金(批准号:2987519)和重庆科技攻关项目(批准号99-5772)资助

摘　　要：应用荧光分光光度计进行共振光散射测定,研究了水相介质中长链烷基化物十二烷基硫酸钠(ＳＬＳ)与牛血清白蛋白(ＢＳＡ)的结合平衡．实验表明,在微酸性条件下,ＳＬＳ与ＢＳＡ形成的复合物引起共振光散射增强．根据４７０ｍｍ的光散射增强信号建立了一定酸度下ＳＬＳ与ＢＳＡ结合的数学关系式．随着ｐＨ值的变化,二者相互作用的结合平衡常数(Ｋｆ)及每分子ＢＳＡ所结合的ＳＬＳ个数(ｎ)均不相同．The binding of sodium laury sulfate(SLS) to bovine serum albumin(BSA) was studied byresonance light scattering in the present paper. It was showed that the interaction results in stronglyenhanced resonance light scattering signals characterized by the are peak at 470 nm.The bindingconstant Kf and the number of binding n were calculated according to the equilibrium equation,which was established based on the data of the resonance light-scattering measurements at 470 nm.It was found that both binding constatn Kf and the number of binding n were changed with varyingpH.

关 键 词：十二烷基硫酸钠 牛血清白蛋白 共振光散射 合成洗涤剂 废水处理 废水监测 

分 类 号：O657.3[理学—分析化学]