己酸正辛酯的反相高效液相色谱法分离检测  

Separation and Determination of Octyl Caproate by Reversed Phase High Performance Liquid Chromatography (RP-HPLC)

作　　者：周国伟[1] 黄锡荣[2] 李越中[3] 李干佐[1] 胡玮[3] 宋少芳[2] 

机构地区：[1]山东大学胶体与界面化学教育部重点实验室,山东济南250100 [2]山东大学化学学院,山东济南250100 [3]山东大学微生物技术国家重点实验室,山东济南250100

出　　处：《精细化工》2001年第1期29-33,共5页Fine Chemicals

基　　金：国家自然科学基金资助项目! (2 990 30 0 6 )

摘　　要：按常规有机合成方法合成了己酸正辛酯 ,采用在KromasilC18(15 0mm× 4 6 0mm ,5 μm)柱上 ,以V(甲醇 )∶V(水 ) =95∶5为流动相及示差折光检测器的反相高效液相色谱法 ,30℃分离和测定了己酸、1 辛醇及己酸正辛酯。在选定的色谱操作条件下 ,三者具有良好的分离效果 ,并求出了三者的标准曲线方程 ,该方法具有较好的准确性和重现性。Octyl caproate was synthesized according to the general organic synthesis.The separation and determination of caproic acid,1 octanol and octyl caproate by high performance liquid chromatography on the Kromasil C18(150 mm×4 60 mm,5 μm) column with V (methanol)∶ V (water)=95∶5 as mobile phase and with refractive index detector were also studied.Under the selected conditions of the chromatographic operation,there was fair separation of caproic acid,1 octanol and octyl caproate.The accuracy and repetition of this method were also good.

关 键 词：反相高效液相色谱法 己酸正辛酯 分离 检测 

分 类 号：TQ225.241[化学工程—有机化工] O657.72[理学—分析化学]