上海地区部分人群输血传播病毒(TTV)感染的研究  

Study on TTV Infecting in Shanghai

作　　者：张家琪[1] 史济弘[1] 王华梁[2] 方芳[2] 朱霄[1] 胡振华[1] 谢凤珍[1] 胡进[1] 于川[1] 

机构地区：[1]上海市杨浦区疾病预防控制中心,上海200090 [2]第二军医大学东方肝胆外科医院

出　　处：《中国公共卫生》2001年第7期581-583,共3页Chinese Journal of Public Health

基　　金：杨浦区卫生局科研资金项目专项基金资助

摘　　要：目的　调查上海地区输血传播病毒 (TTV)在不同人群中的感染情况 ,探索TTV的高危人群和传播途径。方法　应用半巢式PCR方法检测TTVDNA ,并抽取部分阳性标本的PCR产物 (2 49bp)测定核苷酸序列。结果　检测有关人群 8组计 473例 ,其中非甲～非庚型肝炎病人 60例 ,甲～庚型肝炎病人 10 2例 ,血液透析病人 5 5例 ,血液病儿童 38例 ,健康人群 68例 ,以及性病病人、静脉吸毒人群、肝癌病人各 5 0例。TTVDNA总阳性率 2 8 33% (134 /4 73)。阳性率波动在 17 64%～5 0 %。 8组人群中静脉吸毒人群阳性率高达 5 0 % (2 5 /5 0 ) ,性病组阳性率 36% (18/5 0 ) ,该 2组人群TTV感染率与健康人群 (17 64% )相比均有显著性差异 (χ2 =14 0 1P <0 0 0 1,χ2 =5 12P <0 0 5 )。其余各组群阳性率差异比较无统计学意义。将肠道传播途径的组群 (甲、戊肝 )与血液传播途径的组群 (静脉吸毒者、血液病疾患、血液病人及乙、丙肝病人 )比较分析 ,其阳性率分别为 2 5 49%、32 17%。二者无显著性差异 (χ2 =0 46,P >0 0 5 )。 2 0份阳性标本扩增产物测序结果 ,核苷酸序列与日本株 (N2 2 )同源性在 85 %以上的有 15例 ,在 <85 %的有 5例。结论　研究证实上海地区存在较广泛的TTV感染 ,其中静脉毒瘾者与性病患者为高危人群 ,传?Objective To investigate the situation of TTV infecting in various crowds in Shanghai and to explore the high risk crowd and spreading way of TTV.Methods Applying semi nested PCR to test TTV DNA and picking out some PCR outcome(249bp)of positive sample to determine the nucleotidesequence.Results Eight groups counting to 473 are examined,sixty patients are non A～G hepatitis,102 patients are A～G hepatitis,55 patients are hemodialysis,38 children are blood disease,68 healthy men and 50 patients are venereal disease,vein freak out and cancer of the liver.The gross masculine rate of TTV DNA is 28.33%(134/473).The masculine rate fluctuates between 17.64% and 50%.The mascline rate of vein freak out is 50%(25/50)and venereal disease is 36%(18/50),both of which have the notable difference(χ\+2=14.01,P<0 001,χ\+2=5.12,P>0 05)from the healthy men(17.64%).The difference of masculine rate in other group has no statistics meaning.Comparing the group of intestines infecting(A,E hepatitis)and the group of blood infecting(vein freak out,blood disease,hemodialysis and B,C hepatitis),the corresponding masculine rate of which is 25.49% and 32.17% and has no notable difference(χ\+2=0.46,P>0.05).The sequence result of spreading outcome in 20 masculine samples shows that there are 15 samples which the isogenous of nucleotide sequence and Japanese strain(N22) is higher than 85% and 5 samples lower than 85%.Conclusion The research proves that the infection of TTV is rather comprehensive in Shanghai,in which the vein freak out and venereal disease are high danger crowd and there maybe exists spreading way of sex contact and non hematogenous dissemination besides blood infection.TTV is in carrying state in crowd and may be a poor nosogenetic virus.The aberrance of TTV ototoxicosis strain is in existence in Shanghai.

关 键 词：TTV病毒 PCR 核苷酸序列 高危人群 上海 输血传播病毒感染 

分 类 号：R512.6[医药卫生—内科学]