β_3-肾上腺素能受体基因与2型糖尿病关系的研究  

Study on Association of Mutation of the β_3-adrenergic receptor Gene with NIDDM

作　　者：陈玉[1] 周玲[2] 徐耀初[2] 沈洪兵[2] 喻荣彬[2] 钮菊英[2] 

机构地区：[1]扬州大学旅游烹饪学院,扬州225001 [2]南京医科大学公共卫生学院

出　　处：《中国公共卫生》2001年第7期586-588,共3页Chinese Journal of Public Health

摘　　要：目的　探讨 β3 -肾上腺素能受体 (β3 -AR)基因Trp64Arg突变与 2型糖尿病 (NIDDM )有无关联。 方法　对130例糖尿病患者和 130例健康对照者 ,采用聚合酶链反应 -限制性酶切酶片段长度多态性技术检测 β3 -AR基因型 ,并测定BMI、WHR、血压、TC、TG、HDL -C、载脂蛋白A、载脂蛋白B和Lp(a)。结果　 (1)糖尿病病例组β3 -ARArg等位基因频率 (14 7% )略高于对照组 (14 2 % ) ,两组基因型和等位基因频率分布差异无显著性 (P >0 0 5 )。 (2 )糖尿病病例组中Trp64Arg与BMI相关。女性糖尿病人群中 ,肥胖与体重正常者Arg64等位基因频率分别为 19 8%和 5 7% ,该突变与BMI呈显著正相关 (P =0 0 10 )。结论　 2型糖尿病 (NIDDM)患者β3Objective To ascertain the association of the Trp64Arg mutation in the beta 3-adrenergic receptor(β 3-AR)gene with the non-insulin-dependent diabetes mellitus(NIDDM).Methods One hundred thirty patients with NIDDM and 130 controls were selected.The β 3-AR genotype status was determined by polymerase chain reaction (PCR) and restriction fragment length polymorphism analysis(RFLP).Body mass index(BMI),waist hip ratio(WHR),blood pressure,TC,TG,HDL-C,ApoA,ApoB,Lp(a) were detected.Results (1)The frequency of Arg64 allele of β 3-AR gene in NIDDM(14.7%) was slightly higher than that in control(14.2%).Genotypes and the frequency of Arg64 allele do not exist differnece between two groups(P>0 05).(2)The Trp64Arg mutation in the β 3-AR gene was associated with BMI in NIDDM(P=0.0055).The frequencies of Arg64 allele were 19.8% and 5.7% in female diabetes with obesity and normal weight respectively.The mutation exists significantly positive relationship with BMI(P=0.010).Conclusion These results suggest that the Trp64Arg mutation of the β 3-adrenergic receptor gene in the non insulin dependent diabetes mellitus is associated with obesity.

关 键 词：Β3-肾上腺素能受体基因 体质指数 糖尿病 肥胖 

分 类 号：R587.1[医药卫生—内分泌]