离体培养西葫芦子叶花芽分化最佳龄期的选择  

Studies on the Floral Bud Differentiation of Cotyledon of Cucurbita Pepo L. in Vitro

作　　者：黄作喜[1] 沈惠娟[2] 谢寅峰[2] 

机构地区：[1]云南省农科院园艺所,云南昆明650205 [2]南京林业大学,江苏南京210037

出　　处：《南京林业大学学报（自然科学版）》2001年第6期15-18,共4页Journal of Nanjing Forestry University：Natural Sciences Edition

基　　金：国家自然科学基金资助项目(39770604)

摘　　要：为研究花芽分化的机理，选择西葫芦种苗在一片真叶期时的子叶为外植体，培养于附加ＫＴ１．０ ｍｇ／ＬＭＳ培养基中，其花芽分化率可达 ６０％；分析西葫芦种苗在一片真叶期时子叶内源激素和多胺含量，发现子叶内源 ＧＡ１／３含量偏低，而 ＺＲｓ含量剧增，同时 ＺＲｓ／ＧＡ１／３、ＡＢＡ／ＧＡ１／３、Ｓｐｄ／Ｐｕｔ、Ｓｐｍ／Ｐｕｔ比值较高。这些内源因素有利于西葫芦子叶花芽分化。We chose the cotyledous of cucurbit seedlings during first real leaf emerged as explant. KT 1.0 mg/L as a ideal hormone treatment in which a floral experimental system of cucurbit cotyledon was established in vitro. This system showed good repeat and higher ratio of floral bud differentiation (60%). The content of endogenous GA1/3 in the cotyledons is lower during first real leaf of cucurbit seedlings, but ZRs increased significantly, meanwhile ZRs/GA1/3 ABA/GA1/3 Spd/Put Spm/Put showed higher ratio which benefit floral bud differenfiation of cotyledons. So that it is ideal seedlings age to cut cotyledon during first real leaf emerged of cucurbit seedlings.

关 键 词：花芽分化 西葫芦 子叶 离体培养 内源激素 多胺含量 龄期 成花机理 

分 类 号：Q945.64[生物学—植物学] Q943.1