花生白绢病的温室接种技术及抗性鉴定  

Inoculation Techniques and Evaluations of Resistance to Sclerotium rolfsii in Peanut in Greenhouse

作　　者：董炜博[1] 石延茂[1] 孙爱香 耿英修 刘甲玉[3] 

机构地区：[1]山东省花生研究所,山东青岛266100 [2]山东省莱西市植保站 [3]山东省农药检定所

出　　处：《花生科技》2001年第3期17-20,共4页

摘　　要：1999～ 2 0 0 0年 ,在温室内采用碎叶接种法 (A)、竹夹接种法 (B)、牙签接种法 (C)、菌丝块贴茎接种法 (D)、成熟菌核接种法 (E)和萌发菌核贴茎接种法 (F)等 6种方法对花生进行了接种白绢病菌 (Scle rotiumrolfsiiSacc.)试验 ,结果表明 ,以竹夹法 (B)、牙签法 (C)菌丝块贴茎法 (D)及萌发菌核法 (F)的接种发病效果较好 ,可以作为花生白绢病抗性温室鉴定的接种方法 ;而采用碎叶法接种 ,病害的发展有一个停顿 ,成熟菌核法病情发展缓慢 ,均不宜作为花生抗白绢病温室鉴定的接种方法。温室内对鲁花 9号等 1 1个花生品种 (系 )采用菌丝块贴茎接种法进行抗病性鉴定的结果显示 ,参试材料中只有白沙 1 0 1 6对白绢病表现出一定的抗性 ,其它品种 (系 )During 1999-2000, six inoculation techniques of peanut with Sclerotium rolfsii Sacc. were tested in greenhouse. Inoculation techniques for the experiments were (A) smashed peanut leaf technique; (B) bamboo clip technique; (C) toothpick technique; (D) mycelia plugs clasp stem technique; (E) mature sclerotia clasp stem technique; and (F) germinating sclerotia clasp stem technique. The results shown that (B) bamboo clip technique; (C) toothpick technique; (D) mycelia plugs clasp stem technique; and (F) germinating sclerotia clasp stem technique were the most effective methods. The smashed peanut leaf technique (A) and the mature sclerotia clasp stem techniques (E) had obvious disadvantages to inoculate peanut in greenhouse for resistance evaluation. We prefer the mycelia plugs clasp stem technique for screening of individual plant resistance because it is reliable and easy. It indicated that only Baisha 1016 was partial resistant and the other 10 tested peanut varieties were middle or high susceptible to stem blight.

关 键 词：花生 白绢病 鉴定 抗性 温室 接种技术 

分 类 号：S435.652[农业科学—农业昆虫与害虫防治] S565.2[农业科学—植物保护]